I want to preserve tissues from plaque in peyronie's disease. Then I will do primary culture. Is this possible, does it preserve cell viability? I store the tissues in the RNA save solution. How will cell viability be when dissolved? has anyone tried it?
Hello Ebru Derici
Yes, it could be possible to generate cell line from frozen tissue if the tissue is frozen in a way that enables the recovery of viable cells. Just as the mammalian cells are cryopreserved in cryoprotectant like DMSO, so also if the tissue is cryopreserved as small pieces in 10% DMSO + 90% culture medium, it is possible to generate a cell line from frozen tissue. Just keep in mind that you thaw the tissue as quickly as possible very much similar to the protocol followed in thawing mammalian cells. The thawed tissue can then be processed to generate the cell line in a way you would do for the fresh tissue.
Do not store the tissues in RNA save solution, but instead use 10% DMSO + 90% culture medium as it would help to preserve cell viability.
Best.
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