Is it possible to fuse protoplasts of Chlamydomonas with protoplasts of Arabidopsis?

More Peter Rehbein's questions See All

Can anyone report on the molecular weight of Q5 Polymerase?

We are repeatedly troubled with strange Protein bands in PAGE after PCR using Q5 polymerase from NEB. Who has measured the molecular weight of Q5 already and could help us out? Thank you all in...

31 December 2019 6,048 3 View

Who can help in collecting export files of fluorescence spectrometers?

I am writing a script for Processing fluorescence spectra. In Order to make it useful for a large audience, I am collecting Sample Data of Export Files from fluorescence spectrometers of different...

03 April 2018 8,105 11 View

New inducer molecule - which journal to publish?

Dear all,I was able to identify a new, synthetic molecule which is able to induce expression in bacterial gene regulation (only a particular operon is effected) - The practical use of this...

11 December 2016 7,220 0 View

Ion exchange chromatography strategy?

Dear all, after IMAC elution, my E. coli expressed protein (pI=6,32, MW=13 kDa) is in 400 mM Imidazol and 0,3 M KCl. TEV-Protease and cleaved off His-Tag are also swimming in this solution. I want...

05 June 2016 3,911 12 View

Strange Gel-Permeation Chromatogram?

Dear all, my latest gel permeation purification produced some strange results (see chromatogram attached).I am purifying a small 14 kDa protein after Ni-NTA-affinity chromatography and subsequent...

04 May 2016 1,212 7 View

Lysate way too viscous, impossible to apply to affinity-column?

Dear all, my E. coli lysate is extremely viscous, to the point that it is impossible to apply it to the column for purification. What can I do?I added 500 units of DNAse to 50 ml, but the...

04 May 2016 9,746 8 View

Fungal contamination of E. coli culture?

Dear all, lately I get some serious contamination in my E. coli cultures. I suppose it is a fungus of some kind. I am using minimal medum with no trace elements and no vitamins, the only organic...

04 May 2016 9,078 46 View

Problem with protein dimerization?

Dear all,I am trying to purify recombinant prion protein (PrP).The chromatogram during gel permeation chromatography shows that nearly half of my protein exists in the fom of dimers. Running...

04 May 2016 10,064 4 View

Ammonium sulfate precipitate does not sediment, but floats?

Dear all, I am using ammonium sulfate precipitation of protein as a first purification step from my crude bacterial lysate.The method works well, the proteins precipitate in sequential order as...

04 May 2016 3,915 9 View

Load prepacked affinity columns fast or slowly with protein?

Dear all, we all use prepacked chromatography columns for affinity binding in protein purification (most prominent metal affinity like IMAC). I always wondered whether it would be better to load...

03 April 2016 644 5 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

Is it common for heterotrophic bacteria to grow on Pro99 and SNAX media?

I am attempting to isolate picocyanobacteria from seawater (pre-filtered with a GF/D membrane) using the pour plate technique and filter plating method (Kearney et al., 2022) on Pro99 and SNAX...

30 July 2024 1,040 1 View

Question about MHB (Mycorrhiza Helper Bacteria) isolation technique??

What are the most effective isolation techniques for obtaining MHB?? Is there are best approach techniques? I keep struggling in obtaining it.

26 July 2024 7,387 0 View

Question about Single Strain Arbuscular Mycorrhiza Fungi??

Now i am working on AM fungi single strain. What are the most effective isolation techniques for obtaining pure cultures of arbuscular mycorrhizal (AM) fungi?? Is there are best approach...

26 July 2024 6,520 1 View

Isolation of PLV6-Bmal-Luc plasmid?

I am currently at a stage in my research where I will be using the pLV6-Bmal-luc plasmid (Addgene #: 68833). However, I am encountering some issues with the isolation of this plasmid and am unable...

23 July 2024 5,296 0 View

What is the function of fusion tag GAMGSGIQRPTST?

I found that several expression constructs of published protein structures containing an N-teiminal fusion tag GAMGSGIQRPTST. What's the founction of this fusion tag?

23 July 2024 1,353 4 View

The best source for amplification of ADAM17 prodomain?

hi every one I am making vector construction (for fusion proteins) and in this moment I wanna to amplification of ADAM17 prodomain with PCR. to yet, I couldn't amplified the ADAM17 prodomain with...

21 July 2024 8,660 1 View

Kalyanaraman Rajagopal

Yes it is possible i think. Think we need a media which supports growth and maximum metabolite production. Novel effort.

Eylem Aydogdu Lohaus

There is this, protoplast fusion of carrot and chlamy..

http://www.ncbi.nlm.nih.gov/pubmed/?term=Transfer+of+organelles+of+the+alga+Chlamydomonas+reinhardii+into+carrot+cells+by+protoplast+fusion