Is it possible to fuse protoplasts of Chlamydomonas with protoplasts of Arabidopsis?

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Can anyone report on the molecular weight of Q5 Polymerase?

We are repeatedly troubled with strange Protein bands in PAGE after PCR using Q5 polymerase from NEB. Who has measured the molecular weight of Q5 already and could help us out? Thank you all in...

31 December 2019 5,875 3 View

Who can help in collecting export files of fluorescence spectrometers?

I am writing a script for Processing fluorescence spectra. In Order to make it useful for a large audience, I am collecting Sample Data of Export Files from fluorescence spectrometers of different...

03 April 2018 7,945 11 View

New inducer molecule - which journal to publish?

Dear all,I was able to identify a new, synthetic molecule which is able to induce expression in bacterial gene regulation (only a particular operon is effected) - The practical use of this...

11 December 2016 7,073 0 View

Ion exchange chromatography strategy?

Dear all, after IMAC elution, my E. coli expressed protein (pI=6,32, MW=13 kDa) is in 400 mM Imidazol and 0,3 M KCl. TEV-Protease and cleaved off His-Tag are also swimming in this solution. I want...

05 June 2016 3,754 12 View

Strange Gel-Permeation Chromatogram?

Dear all, my latest gel permeation purification produced some strange results (see chromatogram attached).I am purifying a small 14 kDa protein after Ni-NTA-affinity chromatography and subsequent...

04 May 2016 1,063 7 View

Lysate way too viscous, impossible to apply to affinity-column?

Dear all, my E. coli lysate is extremely viscous, to the point that it is impossible to apply it to the column for purification. What can I do?I added 500 units of DNAse to 50 ml, but the...

04 May 2016 9,555 8 View

Problem with protein dimerization?

Dear all,I am trying to purify recombinant prion protein (PrP).The chromatogram during gel permeation chromatography shows that nearly half of my protein exists in the fom of dimers. Running...

04 May 2016 9,918 4 View

Fungal contamination of E. coli culture?

Dear all, lately I get some serious contamination in my E. coli cultures. I suppose it is a fungus of some kind. I am using minimal medum with no trace elements and no vitamins, the only organic...

04 May 2016 8,895 46 View

Ammonium sulfate precipitate does not sediment, but floats?

Dear all, I am using ammonium sulfate precipitation of protein as a first purification step from my crude bacterial lysate.The method works well, the proteins precipitate in sequential order as...

04 May 2016 3,536 9 View

Which amino-derivatized sugars are metabolized by E. coli?

Dear all, I wondered which amino-sugars can be digested by E. coli.Sure, Glucosamine can be metabolized by these bugs. But which other amino-derivatized carbohydrates can they devour?What about...

03 April 2016 4,326 4 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

How to improve RNA isolation?

Hi, I have been struggling with a weird issue for my RNA samples. I used the Qiagen RNA plus mini kit. For some reason no matter how careful I am with the washes, when I measure the RNA, I get...

22 February 2021 1,286 6 View

What software is capable of opening a file with the extension ".cad" ?

It seems like quick cad was the software but had been discontinued. I tried with other Autodesk software such as fusion 360, AutoCAD. I also tried with QCAD. Please let me know if you know of any...

22 February 2021 9,634 3 View

What might be the reason for obtaining two bands in plant genomic DNA extraction?

I got a thin band and a thick band i guess it would be the genomic DNA and the 260/280 ratio is 3.

21 February 2021 6,523 6 View

FDC and FRC cell populations in mouse spleen?

I was trying to isolate fibroblastic reticular cells (FRC) and follicular dendritic cells from mice spleen, but kept getting poor yield. I found from literature that the frequency of FDC is around...

17 February 2021 3,506 4 View

Can 5-FOA Diffuse Through Agar?

I am preparing to do a gene knockout in the filamentous fungi Coccidioides posadasii targeting pyrG/URA3 so that 5-FOA can be used for selection pressure instead of traditional antibiotic...

16 February 2021 169 4 View

What is the best technique for combining images?

Hi, Sorry I don't have much experience in image processing. In my research, I need to combine 2 grayscale images(like attached images) into one! I came across the image fusion process. In the...

16 February 2021 7,317 7 View

Does the property of GFP affect a fusion protein's localization?

Hello, I am planning to insert GOI between CaMV 35 promoter and mGFP in pCambia1302 to produce a fusion protein and see subcellular localization of the protein. But the vector says it's membrane...

15 February 2021 4,353 3 View

How to produce huge amount of protoplasts from Fusarium graminearum?

Hi, all I am using some protocol for protoplast production from Fusarium graminearum (Fg), but I need them in a huge amount. Sometimes the protocol worked well but most of the time failed....

14 February 2021 2,286 4 View

Buillding 3D Model Fussion Protein with Modeller?

Hello May i ask you about using Modeller for making 3D model fussion protein. I want to build a fusion/chimera protein structure which is including 2 different protein and then evaluate quality...

09 February 2021 9,369 2 View

Kalyanaraman Rajagopal

Yes it is possible i think. Think we need a media which supports growth and maximum metabolite production. Novel effort.

Eylem Aydogdu Lohaus

There is this, protoplast fusion of carrot and chlamy..

http://www.ncbi.nlm.nih.gov/pubmed/?term=Transfer+of+organelles+of+the+alga+Chlamydomonas+reinhardii+into+carrot+cells+by+protoplast+fusion