I am working on extraction of aflatoxin from sorghum grains and our lab has currently run out of methanol. Is there any other method to extract aflatoxin using water or any other polar solvent?
Water alone is not suitable to extract aflatoxins. However, there are suiitable alternatives without methanol.
Alternative solvent mixtures for extraction of aflatoxins are:
- acetonitrile/water (84 : 16, v/v)
- acetonitrile/water (90 : 10, v/v)
- chloroform/water (30 : 1, v/v)
- chloroform (pure)
- acetone/water (85 : 15, v/v)
- acetone/water (90 : 10, v/v)
- acetone/hexane/water (54 : 44 : 2, w/w/w)
You should take into account that solvents like acetone or acetonitrile are more “aggressive” than methanol and you have to use appropriate/resistant laboratory material.
Our multi-toxin method works with acetonitrile/water/acetic acid 79/20/1 (the acid promotes extraction of fumonisins), extraction eficiency for aflatoxin is fine. There is a paper from HAns Mol in Analitcal Chemitry (I thiink from 2008) describing different solvents and their extraction efficienc y for mycotoxins, pesticides and veterinary drugs.
There are many of protocols fro Aflatoxin extraction , you should chose the protocol depend with what solvent you have in the lab, also is depended in which method you will use to detection aflatoxin.
what about acetone,
is there any methot uses aceton in elution of Aflatoxins from IAC by aceton ??
There are so many methods. You have to choose one which suits your sample type.
Of curse, you can use acetone 70% in water good extraction solvent. İf you like chloroform can be used. In the case of chloroform, the substrate must be wet.
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