It sounds interesting,  but how could be possible create a double layer of phospholipids around  a gas particle, where also water molecules need to be included ?

Hai Paolo Trucillo, Go through the article which i have attached below.

Hai Zhengwei Huang, do you have any interest on this, if so kindly give me more details on this..

Hi, thank you for sharing this paper. I read it, but still it is not clear to me what is the preparation method of liposomes. As it is said in the paragraph "LOM suspension preparation and characterization", a combination of different PEGilated lipids and surfactants were hydrated in 0.9% sodium chloride. LOMs were manufactured with a customized apparatus that included sonication, size isolation by flotation, and concentration by centrifugation."

I am interested in this field because I have never heard about gas entrapped particles in the DDS systems.

About liposomes, generally the lipidic layer is self-assembled around a water droplet, but how could this happen spontaneously around a gas particle?

Hi,

In addition to the ingredients and type of liposome / nanoliposome employed (unilamellar, multilamellar, etc.), the parameters influencing manufacture and stability of the mentioned product are:

*Type of surfactant(s) / emulsifier(s) used;

*The equipment used to generate gas bubbles of right size and speed / rate of bubble generation;

*The suspension media (pH, osmolarity);

*Process temperature;

*agitation speed;

*Rate and sequence of introducing surfactants / emulsifiers and ingredients of liposomes to the system;

*Polymers / any other ligands used to cover the surface of liposomes externally;

*...

I hope these explanations help; otherwise let me know.