I would like to do a stain similar to the one described in:
J Histochem Cytochem. Jun 2008; 56(6): 539–550.
doi: 10.1369/jhc.2008.950246PMCID: PMC2386766A Rapid Method Combining Golgi and Nissl Staining to Study Neuronal Morphology and Cytoarchitecture
I have freshly frozen sections from a mouse brain.
Hello,
See de comentaries of Firendland et al., 2006 "A modified Golgi staining protocol for use in the human brain...".
But... you can defrost the frozen section with buffer's wash, and continue from "Rapid Golgi Staining" of Pilati et al., 2008 (same as you quotaded).
see to this artical 'Quick Golgi method: Modified for high clarity and better neuronal anatomy' by patro et al 2013 if can work with few modification while fixation of slides in stain in coplin jar. i think this will be helpful and can give you one good publication also. try and validate this. best of luck its freely available on net http://nopr.niscair.res.in/handle/123456789/21074
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