Alison Blain

8 Questions 21 Answers 0 Followers

Questions related from Alison Blain

Hi there. Does anyone know of a way to quantify changes in Golgi morphology in myoblasts?

The myoblasts have been stained with a Golgi marker and DAPI

08 August 2017 5,199 0 View

Why are some mice more awkward to occlude for PV loops?

I work with mouse models of duchenne muscular dystrophy and often find that I have more difficulty in obtaining a good VC occlusion in these mice. By 'good' I mean that the loops move in a...

10 October 2014 1,306 5 View

Can anyone help me with erratic ECG problems during MRI scanning in mice?

We use a custom made cradle with 2 leads for ECG, respiration and temperature monitoring during cardiac MRI in our mice. We use the PC-SAM computer interface. We have been having several...

10 October 2014 8,461 3 View

Anyone have an automated method for counting cells/determining cell density on stained brain slices?

I have cresyl violet stained brain sections and would like to look at cell density in different regions of the brain including the hippocampus and areas of the motor cortex. In some places the...

05 May 2014 9,224 8 View

Is it possible to do a golgi stain on freshly frozen sections?

I would like to do a stain similar to the one described in: J Histochem Cytochem. Jun 2008; 56(6): 539–550. doi: 10.1369/jhc.2008.950246PMCID: PMC2386766A Rapid Method Combining Golgi and Nissl...

03 March 2014 5,727 2 View

Is it possible to 'over transfer' proteins from a gel to a membrane (western blotting)?

We were wondering if it is worth transferring a very large protein overnight. I was always led to believe that your proteins can migrate all the way through the membrane and be lost but someone...

02 February 2014 9,079 27 View

ELISA works for spiked TE buffer but not spiked tissue lysate- potential ways to fix this?

I suspect proteins in the lysate are interfering with antibody binding.

01 January 2014 1,311 2 View

Can anyone tell me if there are any hallmark features of kidney or liver damage (in mouse) which can be viewed on histology?

Are there any specific stains I can use? Or do I need to look for markers of damage in blood? I want to assess toxicity of a compound.

10 October 2013 4,383 1 View