Can anyone help me with erratic ECG problems during MRI scanning in mice?

09 October 2014 8,404 3 View

Why are some mice more awkward to occlude for PV loops?

09 October 2014 1,253 5 View

Anyone have an automated method for counting cells/determining cell density on stained brain slices?

I have cresyl violet stained brain sections and would like to look at cell density in different regions of the brain including the hippocampus and areas of the motor cortex. In some places the...

04 May 2014 9,163 8 View

Is it possible to do a golgi stain on freshly frozen sections?

02 March 2014 5,678 2 View

Is it possible to 'over transfer' proteins from a gel to a membrane (western blotting)?

We were wondering if it is worth transferring a very large protein overnight. I was always led to believe that your proteins can migrate all the way through the membrane and be lost but someone...

01 February 2014 9,036 27 View

ELISA works for spiked TE buffer but not spiked tissue lysate- potential ways to fix this?

I suspect proteins in the lysate are interfering with antibody binding.

31 December 2013 1,261 2 View

Can anyone tell me if there are any hallmark features of kidney or liver damage (in mouse) which can be viewed on histology?

Are there any specific stains I can use? Or do I need to look for markers of damage in blood? I want to assess toxicity of a compound.

09 October 2013 4,330 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View

Is there an appropriate CD8 antibody for IHC-P for staining human breast cancer tissues?

Hello, We are planning on staining Formalin-fixed paraffin breast cancer tissue sections (FFPE) with CD8 Ab via immunofluorescence (Tissue IF). Based on our existing protocol and imaging...

24 February 2021 9,578 4 View

Reagents for histology slides?

Dear all, I am planning to study histology and want to do that at home. I have only stained a blood smear with Giemsa but now want to try a wider range of animal tissues. What reagents will I...

24 February 2021 5,430 3 View

Are there lab markers/pens resistant to Roti-Histol?

Hi, I'm looking for a pen/marker to write on the smooth, transparent (non-frosted) part of microscopy slides which does not get washed away by alcohols and most importantly Roti-Histol. I want to...

24 February 2021 9,902 5 View