The myoblasts have been stained with a Golgi marker and DAPI
I work with mouse models of duchenne muscular dystrophy and often find that I have more difficulty in obtaining a good VC occlusion in these mice. By 'good' I mean that the loops move in a...
09 October 2014 1,343 5 View
We use a custom made cradle with 2 leads for ECG, respiration and temperature monitoring during cardiac MRI in our mice. We use the PC-SAM computer interface. We have been having several...
09 October 2014 8,499 3 View
I have cresyl violet stained brain sections and would like to look at cell density in different regions of the brain including the hippocampus and areas of the motor cortex. In some places the...
04 May 2014 9,276 8 View
I would like to do a stain similar to the one described in: J Histochem Cytochem. Jun 2008; 56(6): 539–550. doi: 10.1369/jhc.2008.950246PMCID: PMC2386766A Rapid Method Combining Golgi and Nissl...
02 March 2014 5,758 2 View
We were wondering if it is worth transferring a very large protein overnight. I was always led to believe that your proteins can migrate all the way through the membrane and be lost but someone...
01 February 2014 9,118 27 View
I suspect proteins in the lysate are interfering with antibody binding.
31 December 2013 1,343 2 View
Are there any specific stains I can use? Or do I need to look for markers of damage in blood? I want to assess toxicity of a compound.
09 October 2013 4,412 1 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...
02 August 2024 6,735 1 View
Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...
29 July 2024 8,624 1 View
I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...
29 July 2024 6,484 5 View
Greeting How many events should be counted for the analysis of apoptosis with nuclear staining with Hoechst 33342 whit microscopy ? Are 100 events count sufficient and three repetitions for...
25 July 2024 7,520 0 View
Hi there! I'm hoping to to look at co-localisation of cytokines and markers of different brain cell types (e.g., neurons, astrocytes, microglia) in juvenile rat brains (postnatal day 28) that have...
23 July 2024 2,844 1 View
Hello, I recently stained my cells with an Arf6 antibody. The healthy control looks pretty normal, while the disease group cells have abnormally large black structures in the cell body. Has anyone...
21 July 2024 1,044 1 View
