Is it possible to distinguish monocyte and macrophage using anti CD68 marker BY immunohistochemistry method?

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In comparing the two different species from the same family, would it make sense to include an out-group in the phylogeny tree?

I am working with trematode parasite and my main aim is to confirm the species using molecular approach and see our species is how close to the other reported similar species. I applied to...

02 March 2015 8,855 18 View

What is the best product size of SYBR Green Real-Time PCR to amplify genomic DNA?

Do you think I can make the product of 300bp to 400bp with SYBR Green? I know it is usually less than 200bp in various other purposes. Here I don't want to analyze the copy number, just want to...

08 September 2014 418 11 View

Can anyone suggest which molecular phylogenetic method would be the best to represent a trematode parasite?

Hi, I want to build a phylogenetic tree of ITS2 & 28S r DNA gene partial sequence of trematode parasite by distance and character based method (such as Neighbor-joining (NJ), Maximum...

07 August 2014 3,145 7 View

What is the best marker for macrophage, neutrophils, and plasma cells ?

What is best marker to analyze macrophage, neutrophils and plasma cells in parasite (Trematode) induced granuloma in the human eye by immunohistochemistry methods?

06 July 2013 9,810 13 View

Can someone suggest a specific marker for inflammatory immune cells in parasite (trematode) induced granuloma in the human eye?

I want to characterize the inflammatory immune cell in the parasite (Trematode) induced granuloma in the human eye such as T lymphocytes, B Cells, Eosinophils, Macrophage and Neutrophils, using...

04 May 2013 9,932 1 View

Can I change concentration and brand of antibodies mid-experiment?

I am doing immunohistochemistry of the c-Fos protein and I have a budget problem. For context, the experiment has been done over 3 years prior to my arrival and I have 40 brains done and 100 left....

29 July 2024 5,961 2 View

Sodium azide in immunohistochemistry?

Hello everyone, A collaborator sent us his protocol in order to be able to use his immunohistochemistry kits. But one step contains PBS + 0.3% H2O2 + 0.1% sodium azide. Knowing the dangerousness...

23 July 2024 6,314 3 View

How to label synapses in over-fixed mice brain sections (40 um) via immunohistochemistry (IHC)?

We have mice brains that were over-fixed due to old PFA used during perfusion. Thus, the synapses are no longer being labeled by the Synaptophysin (SY38) mAB. which works perfectly every other...

17 July 2024 7,767 3 View

Is perfusion required to stain brain tissue with every method?

I'm looking to immunostain some brain tissue collected previously. The animal was *not* perfused prior to tissue collection. Will the presence of blood interfere with all staining methods...

14 July 2024 177 4 View

Can I use BSA for a Histological Blocking Solution?

Hi all, I would like to know if it is possible to use Bovine Serum Albumin (BSA) in my blocking solution for immunofluorescence muscle fiber typing. The primary antibodies' host is mouse. The...

07 July 2024 7,967 3 View

To what degree does the immunohistochemical (IHC) evaluation of Ki-67 hold diagnostic value beyond neoplastic pathologies?

In the context of doctoral research, I utilized immunohistochemical (IHC) analysis to quantify the expression levels of the Ki-67 proliferation marker. While the application of this test and...

25 June 2024 7,825 2 View

Help with mounting meningeal tissues with Prolong Diamond?

Hello! I've recently been trying to optimise immunofluorescent staining of mouse meningeal tissue, and I'm having trouble mounting it just because it's so thick. We use prolong to mount and...

05 June 2024 624 2 View

Hello! Does someone know of any antibody for neurotensin?

I need to do immunohistochemistry in mouse brain, particularly in neurotensin-cre mouse line.

22 May 2024 3,565 7 View

Air bubbles forming underneath tissue during dehydration step of IHC?

Hi all, I've processed my own IHC for many years and never run into this problem before, but the last few times I've tried staining, I've had the same issue. I'm wondering if it's a reagent...

08 May 2024 3,979 1 View

Shandon Sequenza leaves my IHC samples with spots that have not been stained?

I have been using Shandon Sequenza for the last two years with no problems. Lately i have noticed small spots under a microscope that have not been stained by DAB (only hematoxylin as a contrast...

28 April 2024 2,530 1 View

Caroline Elizabeth Dunk

CD68 is tricky mostly because it is intracellular so not a good marker. I would instead use CD14 to mark monocyte and a more mature mac marker such as CD206 for classical activation or CD163 for alternate activation M1 vs M2.

hope this helps Caroline

Lalan Kumar Arya

Thanks a lot Caroline.