In comparing the two different species from the same family, would it make sense to include an out-group in the phylogeny tree?

I am working with trematode parasite and my main aim is to confirm the species using molecular approach and see our species is how close to the other reported similar species. I applied to...

02 March 2015 8,624 18 View

What is the best product size of SYBR Green Real-Time PCR to amplify genomic DNA?

08 September 2014 324 11 View

Can anyone suggest which molecular phylogenetic method would be the best to represent a trematode parasite?

Hi, I want to build a phylogenetic tree of ITS2 & 28S r DNA gene partial sequence of trematode parasite by distance and character based method (such as Neighbor-joining (NJ), Maximum...

07 August 2014 3,048 7 View

What is the best marker for macrophage, neutrophils, and plasma cells ?

What is best marker to analyze macrophage, neutrophils and plasma cells in parasite (Trematode) induced granuloma in the human eye by immunohistochemistry methods?

06 July 2013 9,718 13 View

Can someone suggest a specific marker for inflammatory immune cells in parasite (trematode) induced granuloma in the human eye?

I want to characterize the inflammatory immune cell in the parasite (Trematode) induced granuloma in the human eye such as T lymphocytes, B Cells, Eosinophils, Macrophage and Neutrophils, using...

04 May 2013 9,844 1 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

I am looking for an antibody to Arc protein for use in immunohistochemistry with rhesus monkey brain tissue?

Looking for a well-validated antibody to detect Arc protein expression in paraformaldehyde fixed brain tissue using immunohistochemistry. Ideally this antibody would have been published on using...

07 February 2021 8,457 1 View

How should I go about evaluating cytoplasmic and nuclear Arginase-1 activity?

Hello! I've stained some bright field Arginase-1 Hematoxylin slides, but I'm having a hard time finding any computed histology predicates for evaluating the intensity of the cytoplasm verses the...

03 February 2021 3,039 1 View

Antibody dilution for IHQ in OCT frozen tissue is more similar to IHC in Paraffin-embedded samples or to Immunocytochemistry?

31 January 2021 8,678 3 View

Any tips on Fab blocking of mouse tissue?

Hi, everyone, I am working on immunostaining of bone marrow and growth plates of mice. And I have to use an mouse monoclonal Ab to do this staining. However, with only secondary goat anti-mouse...

21 January 2021 9,826 3 View

Antigen retrieval for neural cell marker on frozen brain tissue?

Hi, I'm having problems with the Recombinant Anti-MAP2 antibody from Abcam (Recombinant Anti-MAP2 antibody [EPR19691] (ab183830)) antibody on my frozen, 4%PFA perfused, mice brain tissue. I...

10 January 2021 4,002 5 View

How to approach quantification of cytoplasmic immunofluorescent signal in adipocytes?

Any ideas on how to quantify a non-nuclear immunofluorescent signal in adipocytes? I had the idea to do a watershed on inverted image to "locate" the center of the empty space where lipid droplets...

05 January 2021 5,420 1 View

Can you recommend a protocol for immunohistochemistry of mouse brain sections using anti-MAP2?

Current protocol does not give clear images.

04 January 2021 1,614 3 View

I need to know about immunohistochemistry steps?

Immunohistochemistry staining. Tissue Fixation during IHC. Using DAB. blocking step. controls group.

23 December 2020 5,768 2 View

ISO antibody against BFP for FFPE IHC?

Suggestions and recommendations welcome. Any usage/protocol experience would also be helpful/appreciated. Cheers

22 December 2020 599 3 View