I want to characterize the inflammatory immune cell in the parasite (Trematode) induced granuloma in the human eye such as T lymphocytes, B Cells, Eosinophils, Macrophage and Neutrophils, using IHC-P methods.
For that I need a help from an expert to select a specific marker such as anti CD3, anti CD4,anti CD8, anti CD20 , anti Eosinophils , anti CD68 and anti Neutrophil which is appropriate for parasitic infection.
Is it okay to study the panel of cells at a time or is it better to analyze a few cells to see the cell pattern first?
Please suggest the antibody clone with company name.
Dear Dr. Arya,
It is important to know which kind of material you will be working with before risking any advice.
Do you intend to use frozen sections of the eye regions affected by the granulomas ?
Or do you intend to work with material already fixed in formalin or embedded in paraffin ?
Or do you intend to macerate/ digest the tissue in order to release the cells (complicated, poor yield and may not reflect the tissue.
Do you intend to quantitate the number of cells in sections by the staining of sections with anti-CD4 or anti-CD8 or similar (which is practically impossible) or an evaluation of the stained- area will be sufficient ( OK and within the limitations of IHC method in sections) ?
Ises A. Abrahamsohn , São Paulo , Brazil, Immunology, retired Prof . University of São Paulo.
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