Hi:
Does anyone know if it is possible to detect mRNA expression levels of Tau variants (e.g. Tau40, Tau42) in non-cerebral cell lines such as HUVEC and THP-1? mRNA expression levels can be detected in transgenic mice expressing such human variants, but I am not sure if it possible in cell lines. Given that Tau is a microtubule‐associated protein found in epithelial cells (an other types), it is possible that Tau expression may be up/down regulated depending on the cellular circumstances, but I am not sure if the splicing events related with Alzheimer has been observed in cell lines and detected by qPCR.
Thank you in advance,
Rafael.
Yes, you can detect changes in mRNA levels of tau using real PCR. There are no reports on this topic (to my best knowledge) as of now. But keep in mind that although tau is expressed in many tissues, they vary in that if they predominantly express short type tau or the big tau or both in equally.
- Badges
- Science topic
- Similar topics
- Linguistics
- Composition Studies
- Publication