11 November 2016 1 9K Report

I want to check the expression two miRNA's from stool samples derived from small intestinal tight junction. Other papers have worked with blood and intestinal tissue biopsy. Can miRNA be expressed in stool? 

More Maisha Khair Nima's questions See All
When I try to submit my sequence through Bankit it says the sequence contains vector sequence but it actually doesn't. How do I explain this to NCBI?

I've been trying to submit a sequence at Genbank using BankIt. It's an HRP2 exon2 partial CDS of Plasmodium falciparum. Everytime I paste my sequence it says that my sequence contains vector...

05 June 2016 8,178 3 View

Similar questions and discussions
Anyone having idea about VN primer for miRNA primer design ?

How to design VN primer to attach with universal reverse primer

05 August 2024 2,116 3 View

Which modeling software and force field suitable for miRNA-mRNA molecular simulation?

Hello everyone, I am currently working on miRNA- mRNA interaction study. Further, I would like to do MD simulations for the miRNA-mRNA models. For the Simulation, I have modelled this complex...

24 June 2024 3,507 2 View

Which is the best small Extracellulare Vesicles (sEVs) concentration to obtain enough RNA for miRNA analysis?

Hello to everyone, I'm trying to extrtact total RNA from sEVs. I made a first experiment starting from 2x10^10 sEVs quantified by NTA but the RNA obtained was very low (under 20ng/ml) Do you have...

18 June 2024 2,799 4 View

What is the best way to synthesize cDNA of miRNA; using poly A kit or stem loop PCR?

Recently, I have been trying to quantify the expression of miRNA. I have extracted the miRNA. However, I am not clear about using the cDNA synthesis kit usually there are two kits providing by...

16 June 2024 9,723 2 View

Extraction RNA with miRVANA kit?

Hello everyone, I would like to know if any of you ever tried to assess the yeld of RNA extraction with the mirVana™ miRNA Isolation Kit, without phenol from ThermoFisher. I am experincing some...

06 June 2024 5,715 4 View

How to derive the tight-binding Hamiltonian by placing atomic orbitals at Wyckoff positions ????

Dear all, I am new to theoretical physics of condensed matter physics. I am trying to write down a hamiltonian to fitting my first principle calculation. I am confused how to get the hamiltonian...

05 June 2024 9,668 0 View

I need guidance how to design primers for miRNA?

The size miRNA is 89 bps and the primers I have designed (using traditional method for designing gene primers) should give an amplified product of 71 bps. But I am getting product of higher...

23 May 2024 7,570 3 View

Should the target miRNA level decrease after the miRNA inhibitor transfection when checked with qPCR??

I have a question regarding target miRNA expression after transfection with antisense miRNA (miRNA inhibitor). I understand that the expression of the target miRNA will increase after mimic...

20 May 2024 5,920 4 View

Webserver for identifying activation and repression of TFs, genes, and miRNAs?

Hello. Is there any webserver where I can input a set of transcription factors, genes, and miRNAs, and it can provide me with statistical data on how TFs either activate or repress genes and...

18 May 2024 5,848 1 View

How can I determine the width and length of graphene nanoribbons using tight-binding or DFT models?

For easily able to understand I uploaded a image.

15 May 2024 9,768 2 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close