Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Plant research: Watering uniformity/consistency. How important is it?

Trying to gather insight from an unresolved discussion with a colleague here, and either the available literature surrounding this topic and/or my keywords in search of are lacking. Context:...

21 February 2021 6,389 3 View

How to get rough estimation of the concentrations of Oxygen and moisture inside the glove box?

Dear colleagues, I was just wondering if you can suggest a good method to get a rough estimation to the concentration of O2 and H2O inside the glove box as unfortunately, our glove box doesn't...

17 February 2021 1,012 3 View

Onset of Photo Oxidation in Polymers?

Hi! I am looking for some specific information in regards to the minimum amount of UV intensity required to begin the photo degradation process in polymers. I understand each polymer is...

15 February 2021 7,703 4 View

Are you looking for a quality online conference on teaching and learning technology?

This large international conference will be held online this year due to COVID. I am submitting a paper to the Technology Enhanced Language Learning track (8). It is about a topic I have never...

15 February 2021 1,975 15 View

How do you calculate a discrimination index for polytomous data?

Hello, I'm currently running a PCA on a polytomous data set (5 point Likert). I want to create a discrimination index to narrow down my items before running the PCA. After looking around, I'm...

13 February 2021 3,517 2 View

Regarding immunology, transmissivity, herd immunity and vaccines in Sars CoV-2?

This is a follow-up on an earlier question regarding mechanistics of vaccines: why current vaccines don't block transmission. In summary, they don't engage mucosal immune cells to produce IgA....

08 February 2021 8,321 3 View

Which tropical country is the most easy for obtaining research permit (for biological research)?

Dear all, High beurocracy hihly complicate field ecolocial and evolutionary research nowadays. Nearly all tropical countries (maybe all?) requires research permit for performitng research and in...

04 February 2021 3,672 6 View

Can I polymerize NIPPAm with PEGmethacrylate or only with PEGacrylate?

I want to perform the synthesis of a hydrogel and I want to know if I can polymerize NIPPAm with PEGmethacrylate or only with PEGacrylate?

02 February 2021 2,106 6 View

What is the maximum storage time for samples used in soil enzyme assays?

01 February 2021 4,616 3 View

How is the quality of archived blood sample for HbA1c test?

HbA1c is an important indicator of long-term glycemic control with the ability to reflect the cumulative glycemic history of the preceding two to three months. Also, HbA1c is measured as the ratio...

16 September 2020 2,423 4 View

Panel of serum or plasma with antibodies to known pathogens?

Does anyone know of a panel of serum/plasma with antibodies to known pathogens such as Influenza A, HBV, HCB etc, that could be purchased for testing? or through ethics application to biobank?

03 September 2020 3,017 1 View

Who has high quality matched sets of human tissue and biofluids?

Hi, I'm looking for a good resource/company that offers high-quality matched sets of custom human tissue and biofluids. Does anyone know of any good companies that offer these products to...

05 June 2018 1,734 1 View

What are some examples of the most useful research findings to come out of big data and/or biobanks in recent years?

Research and Data analysis on large data sets holds great promise. What have been some of the major findings using big data research in recent years?

07 August 2016 8,037 0 View

Where can I get metastatic cell line MHCC97?

Does anybody know companies/institutes/biobanks that distribute this cell line? Also, if anyone can suggest cell lines with aggressive/metastatic properties, that will be really helpful.

31 December 2015 8,804 1 View

Can Human Beings be produced in large numbers?

Removing the emotions and feelings, which people own or be possessive with being protected and cared during the process of upbringing, is there anything which is akin to mass production of human...

31 December 2015 617 43 View

Could anyone share their experience of using the Fluidx or Micronic 2D barcoded tubes?

02 November 2015 2,615 2 View

Has anyone developed a protocol for the collection of secretory proteins from whole tissue without tissue homogenization and cell lysis?

25 August 2015 1,105 5 View

Has anyone developed a protocol for the collection of secretory proteins from whole tissue without tissue homogenization and cell lysis?

07 August 2015 2,782 6 View

How can I isolate the buffy coat and plasma in the same tube for biobanking?

How to isolate the buffy coat (for DNA extraction) and plasma (for future biomarker discovery) in the same tube for biobanking?

01 February 2015 9,753 2 View