How can I calculate ddCt in my results (real-time PCR results)?

I'm analyzing the results of SYBR green realtime PCR, but I had difficulty in interpreting the results of one of the genes tested.. As shown below, there was no gene expression in the control...

02 June 2019 5,723 2 View

How do I calculate output of solar panels for a given area?

Hi, I'm a little unsure if I have the correct formula. I'm looking to calculate the potential maximum output for Photovoltaic material when I know the surface area. i.e. peak= Wm^2 x total area...

02 March 2019 9,949 4 View

What is the role of the copper precursor in chemical nanoparticle synthesis?

I have been trying to obtain copper nanoparticles in aqueous colloidal suspensions following procedures found in the literature, only I am using copper sulfate instead of copper chloride as Cu2+...

25 January 2018 8,627 3 View

Questions about drawing graphics from sequencing results.

I plan to carry out sequencing of a bacterial integron using random primer walking to identify the structure of internal genes.Is there a program on ncbi homepage that can draw graphics of gene...

23 July 2017 6,279 3 View

Does internal and external dysbiosis increase the risk of atopic disease?

14 February 2016 3,184 3 View

Can anyone suggest me some papers related to the risk factors of the Haemorrhagic Fever with Renal Syndrome caused by Apodemus flavicollis?

Can anyone recommend me some papers related to the risk factors of the Haemorrhagic Fever with Renal Syndrome caused by Apodemus flavicollis?

04 February 2015 2,995 3 View

What is the number of mammalian cells required for phage display?

I am looking to do phage display using a mammalian cell line as the immobilised target and was wondering how many cells I need to use. I have seen a whole range in the literature, but a number of...

09 November 2014 939 4 View

Has anyone tried to increase the Tm of a forward primer with additional random primers at the 3' end? I'm trying with 6N, but it's not working.

I'm trying a gradient PCR to check the best T, previously I have been using 62 as the best option. I would like to increase it to get closer to the Reverse primer, that is 71 degrees. I am using...

13 December 2012 9,012 15 View

How to prevent Uranyl Acetate precipitation?

Hi everyone, I met a problem recently and really need your advise. In the last two rounds of sample embedding for TEM with resin, we found uranyl acetate precipitation right away after sectioning...

01 January 1970 9,263 3 View

Is there a format that I follow to introduce an incubation idea?

I am very new to this project but very excited about collaboration in a global context. Is there a particular format you use that I can prepare a presentation on Creativity? Patricia

01 January 1970 421 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

How does one access the Cranet survey data on HRM practices?

Hello, The Cranet website does not provide any lead to the data obtained from their multinational survey on HRM practices. Any help on accessing the data would be most appreciated. Thank...

01 March 2021 9,536 3 View

Is there any research regarding how training and development is going to change in the era of Fourth Industrial Revolution?

As the organizations getting prepared for the fourth industrial revolution, to enhance the skills of their workforce what type of changes are necessary and going to occur in the training and...

01 March 2021 3,199 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View