What is the reason of not getting any band in SDS- PAGE?

I am doing an experiment on proteins. I precipitated protein using TCA- acetone method and want to check purity on SDS-PAGE. I used 25-30 microgram protein for one well, after running SDS- PAGE,...

24 November 2020 8,885 3 View

How can I study the microstructure of carbon steel C1010 by etching process?

How can I study the microstructure of carbon steel C1010 by etching process. Which chemicals I should use for etching of carbon steel?

07 July 2020 5,106 7 View

Hello all, which machine learning algorithms will be best fit for csv or text datset?and also is that a good idea to use Deep learning on textdaset?

I m working on my dessertation using AI methodology in MATLAB.I have a text dataset so need some suggestions.

19 May 2020 7,862 19 View

My Research paper has published in IJEAT Vol9 Issue1S3Dec 2019. I want to know when will be the Indexing of Research papers in the database of scopus?

My Research paper has published in IJEAT Vol9 Issue1S3Dec 2019. I want to know when will be the Indexing of Research papers in the database of scopus?

23 February 2020 4,006 3 View

Please advise the CSR actvities for the port industry?

For my research paper.

12 June 2019 8,408 6 View

How Bacteria changes the pH of nutrient broth media?

Bacteria can change the pH of media by producing EPS. In my work, initial pH of nutrient broth is 6.5 and after the bacterial growth pH increases about 7.5. Which type of reactions can involved in...

24 February 2019 6,508 4 View

Which rotary evaporater is best for plant extracts?

We want to purchase a rotary evaporater for our lab for evaporating the solvents from plant extracts so can you please suggest me which one is best and easy to handle.

27 May 2018 234 10 View

Which wavelength correseponds to Fe2+ and Fe3+ peaks UV spctrum?

I am doing UV spectroscopy (200 to 700nm) for FeSO4, Ferric sulphate and ferric chloride to find the peaks of ferrous and ferric. but i got peak at 223 to 224nm in all cases. however if compound...

09 January 2018 6,912 7 View

I want to ask should i use different antibiotics for gram positive and gram negative strains?

I am working on antibacterial activity of wild plants of family Asterceae so Iam using 3 gram positive strains and 3 gram negative strains so i want to ask whether i use a single broad spectrum...

27 October 2017 8,402 6 View

Can anyone provide me the protocol of sequential extraction method for plant extraction?

I want to know about the sequential extraction by non polar to polar solvents in Soxhlet apparatus?

11 October 2017 6,664 7 View

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02 March 2021 3,060 3 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

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01 March 2021 3,905 5 View

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28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Can random sampling have inclusion criteria?

As random sampling means that everyone in the population have an equal chance to be chosen, can it have inclusion criteria? My research participants have been chosen based on the inclusion...

26 February 2021 6,905 10 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Has anyone experienced changes in growth for cell culture when purchasing media from different companies?

I recently switched the company where we buy our DMEM/F12 and i have noticed a signficant change in growth for my human olfactory neurosphere cell lines

24 February 2021 2,268 3 View