Is it possible to take a purified virus/particle and cross link to an e.g. agarose bead and then use this bead to deplete serum of antibodies specific to this virus? I have done something similar in the past, cross linking peptides, whole proteins and sugars to beads to purify antibodies, but is it possible with whole virus?
I've never attempted this before, but suspect that it would be less likely to work with enveloped viruses than non-enveloped ones. Also, in principle the only viral antigens exposed for Ab ligation would be ones on the virion surface. So Ab against internal viral proteins would not be removed. Good luck.
I think it can be done. I did similar experiment with 12 nm virus like particle from sulfolobus few years ago in this paper "Something Old, Something New, Something Borrowed;How the Thermoacidophilic Archaeon Sulfolobus solfataricus Responds to Oxidative Stress" 2009 PLOs One September 2009 | Volume 4 | Issue 9 | e6964.
The 12 nm SsDPSL immobilized on the AminoLink Plus Coupling Gel (4% cross-linked beaded agarose, 50% slurry). The DPSL protein was coupled using the pH 10 coupling procedures and using AminoLink Reductant Cyanoborohydride solution
(NaCNBH3). After coupling, the DPSL-agarose beads were mixed with total S. solfataricus total cell lysate in presence of protease inhibitors ...page 4.
Good luck
It should work.
The downside I see is that you can probably only use the beads once because harsh dissociation buffers could destroy the virus.
Also you will elute virus fragments along with your antibodies so an extra protein A sepharose step might be needed to get rid of them.
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