Can the RNASE H1 and H2A proteins be inhibited?

I am looking for a way to inhibit the catalytic function of RNASE H1 and RNASE H2A on the protein level (so not via shRNAs or something similar). Sadly, I just found inhibitors for other...

07 August 2017 6,447 1 View

Is partial reverse transcription of RNA possible?

Assume we are given an RNA sequence of, say, 300 bps. AACAGCUAGCUGAUCGAUGCUAGCUG....ACACUGUACG and now we want to reverse transcribe either the 5' or 3' end in vivo, but not the entire transcript,...

07 August 2017 2,631 3 View

What is the best way to reduce error rates in array synthesized DNA oligonucleotide libraries?

I am looking for ways to reduce the error rates of array synthesised DNA oligonucleotide libraries of, say, approx. 100 000 unique sequences with 170 bps. These have error rates of approx. 0.5%...

10 November 2016 6,751 1 View

Are there good SNP-phenotype association databases?

Currently, I am looking for databases, which associate SNPs and phenotypes (like eye colour or breast cancer risk). These should get updated regularly with regards to recent GWAS etc. Ideally,...

10 November 2016 7,585 10 View

Is there a public patient database for Alzheimer's disease patients?

For a study I would like to monitor the history of Alzheimer's disease patients. Specifically, I would like to address whether which vaccines the patients have received prior diagnoses. If...

05 June 2016 2,272 1 View

Is it possible to remove one strand of double stranded DNA in vivo?

Assume we do have a double stranded DNA sequence in human cells, say ...ATCGATATCGATATTGCAGAGCATAGCTATAA... ...TAGCTATAGCTATAAGCTCTCGTATCGATATT... Now I want to cleave one strand, such that I do...

04 May 2016 4,698 3 View

Separation of smaller DNA fragments and larger fragments without using gel electrophoresis?

For an upcoming experiment I would have two kinds of DNA fragments in a sample after a particular reaction step: First there would be fragments of a length of approx. 170 bps and then there would...

04 May 2016 4,280 5 View

How do I insert DNA barcodes into animal cells and how do I transmit them to the neighbouring cells?

I am currently interested in equipping animal cells in a tissue with individual barcodes. These barcodes should get amplified in the cells (not to a cell degrading extent, of course), and also...

03 April 2016 6,164 0 View

Is it possible the cut the ends of DNA fragments to obtain sticky ends?

Assume we are given a DNA fragment like ACTTAGC....GCTCGGCA TGAATCG...CGAGCCGT so we have blunt ends. I am looking for a nuclease, that cleaves the end, such that we obtain sticky ends. Hence, the...

03 April 2016 8,033 3 View

What is the best way to automatically handle approx. 10 000 frozen DNA/RNA samples and to extract tiny amounts of the samples?

For an upcomig experiment I would have to make use of approx. 10 000 frozen DNA/RNA samples in microtubes, or, if necessary, in well plates. I would have to thaw them gently, to extract approx. 1...

03 April 2016 2,264 11 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Could you please suggests a tutorial or paper or any other materials to learn multiple gene expression in TCGA and GTEx databases?

I would like to analyze multiple gene expressions in normal and cancer tissue by using the data in the TCGA and GTEx databases. Please suggest a tutorial or paper showing step by step analysis of...

23 February 2021 9,007 3 View

Are there any databases for gene co-expression or expression pattern clustering?

I am currently working on gene clustering based on co-expression pattern in mouse brain. The problem is I do not have some solid way to test my result. Are there any suggestions for databases...

22 February 2021 4,374 3 View

Which CpGs have a significant effect on gene expression?

It is known that methylated CpG around TSS decreases gene expression. However, It is difficult to know exactly which CpG has a greater impact on gene expression. Are there any databases known...

20 February 2021 5,634 3 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

In what ways do fellow reaerchers think that mRNA vaccination may be under CRISPR systems technology application ?

There are many ways for mRNA to intervene with genome expression. This question is raised for experts in CRISPR systems. Any RNA does not stand alone within acell. There are many ways to become...

18 February 2021 9,109 5 View

Purification of proteins from baculovirus-infected insect cells?

18 February 2021 6,665 6 View