Tobias A.

13 Questions 5 Answers 0 Followers

Questions related from Tobias A.

Is partial reverse transcription of RNA possible?

Assume we are given an RNA sequence of, say, 300 bps. AACAGCUAGCUGAUCGAUGCUAGCUG....ACACUGUACG and now we want to reverse transcribe either the 5' or 3' end in vivo, but not the entire transcript,...

08 August 2017 2,673 3 View

Can the RNASE H1 and H2A proteins be inhibited?

I am looking for a way to inhibit the catalytic function of RNASE H1 and RNASE H2A on the protein level (so not via shRNAs or something similar). Sadly, I just found inhibitors for other...

08 August 2017 6,487 1 View

Is it possible to co-express a sgRNA (CRISPR/Cas9) and a protein-coding gene?

I want to express a sgRNA for CRISPR/Cas9-based genome editing, which should be expressed at similar levels than a protein-coding gene? Are there any known systems or do you have anything in mind,...

02 February 2017 2,472 2 View

What is the best way to reduce error rates in array synthesized DNA oligonucleotide libraries?

I am looking for ways to reduce the error rates of array synthesised DNA oligonucleotide libraries of, say, approx. 100 000 unique sequences with 170 bps. These have error rates of approx. 0.5%...

11 November 2016 6,798 1 View

Are there good SNP-phenotype association databases?

Currently, I am looking for databases, which associate SNPs and phenotypes (like eye colour or breast cancer risk). These should get updated regularly with regards to recent GWAS etc. Ideally,...

11 November 2016 7,661 10 View

Is there a public patient database for Alzheimer's disease patients?

For a study I would like to monitor the history of Alzheimer's disease patients. Specifically, I would like to address whether which vaccines the patients have received prior diagnoses. If...

06 June 2016 2,302 1 View

Is it possible to remove one strand of double stranded DNA in vivo?

Assume we do have a double stranded DNA sequence in human cells, say ...ATCGATATCGATATTGCAGAGCATAGCTATAA... ...TAGCTATAGCTATAAGCTCTCGTATCGATATT... Now I want to cleave one strand, such that I do...

05 May 2016 4,742 3 View

Separation of smaller DNA fragments and larger fragments without using gel electrophoresis?

For an upcoming experiment I would have two kinds of DNA fragments in a sample after a particular reaction step: First there would be fragments of a length of approx. 170 bps and then there would...

05 May 2016 4,353 5 View

What is the most cost-effective way to generate a DNA oligonucleotide library?

For an upcoming experiment there are situations, where I would have to synthesize 500-5000 different DNA oligonucleotides of a size of 100 bps or less (approx. 60 bps would also be possible) and I...

04 April 2016 4,579 3 View

What is the best was to amplify (small) DNA fragments in a specific sample?

Assume we are given an aliquot with many different double-stranded DNA fragments mostly of size 50 bp - 1000 bp. I would like to amplify all these fragments at once from end to end for each...

04 April 2016 8,296 4 View

What is the best way to automatically handle approx. 10 000 frozen DNA/RNA samples and to extract tiny amounts of the samples?

For an upcomig experiment I would have to make use of approx. 10 000 frozen DNA/RNA samples in microtubes, or, if necessary, in well plates. I would have to thaw them gently, to extract approx. 1...

04 April 2016 2,311 11 View

Is it possible the cut the ends of DNA fragments to obtain sticky ends?

Assume we are given a DNA fragment like ACTTAGC....GCTCGGCA TGAATCG...CGAGCCGT so we have blunt ends. I am looking for a nuclease, that cleaves the end, such that we obtain sticky ends. Hence, the...

04 April 2016 8,070 3 View

How do I insert DNA barcodes into animal cells and how do I transmit them to the neighbouring cells?

I am currently interested in equipping animal cells in a tissue with individual barcodes. These barcodes should get amplified in the cells (not to a cell degrading extent, of course), and also...

04 April 2016 6,201 0 View