I will transform the plasmid that contains the gene of interest. The plasmid will be transformed into yeast cells by electrophoration. The yeast used comes from isolated baker's yeast to obtain a single colony. is it possible for the plasmid to enter into yeast cells? or do I need a competent yeast with a specific strain specifically used for transformation? Please help me.
Michael J. Benedik
The problem is whether you have a selection for the plasmid. The normal lab strains of S. cerevisae are often auxotrophs that are used for plasmid selection but a wild type strain would not have these markers to use. Note that bakers yeast is also still S. cerevisiae but just a notably different strain than the domesticated lab versions.
0 votes
0 thanks
- Badges
- Science topic
More Candra Wahyuningsih's questions See All
Similar questions and discussions