Hy,

you can perform cryosectioning of your lungs, adhere the tissue slices on SuperfrostPlus glass slides. Put them for 15 min at about 35° C on a heat plate to fix them on the slide.

Then fix/incubate in your Bouin solution and continue with your staining.

I think this should work. I did it this way with brain tissue.

Kind regards

Stefan

Stefan Jean-Pierre Haas Thanks for the help. When you fixed with Bouin's after sectioning, did you still achieve the same result of increased contrast between normal tissue and metastases?

Hello,

I never did cancer research. I just used this kind of fixative for neuroscience research. You just should try it with one of your organs and if it works this way, then ok.

Kind regards to Seattle (I was there in 1999 and 2001 for a Summer School in Port Ludlow and had the chance to spend some days directly in Seattle. My hostel was the "green turtle" nearby Pike Market Place)

Stefan

Dear,

I have used frozen specimen for histology. But I was using 10% Neutral Buffered Formalin for the purpose. During my research, the availability of my research samples was at any time and more commonly in late night and it was very difficult to do dissection in odd hours (e.g. 2:30 am etc.). those specimens I was immediately storing in deep freezer at -20oC or below. Then I was thawing those specimen with lukewarm water to bring them at room temperature and in normal consistency. After thawing, I was fixing them in 10% NBF & Davidson's fixative for 24-36 Hrs. or maximum for 72 hrs. Then I was following routine histological processing and sectioning. I have used many staining techniques like H&E, Masson's trichrome, Verhoeff's elastic, Gridley's technique, Mercury Bromphenol blue for protein, PAS-AB for mucopolysaccharides, PTAH, Holmes etc. and did not face any problem with any stain I have used.

But I have never tried it with Bouin's fluid. So if you are planning for Bouin's fluid then you can try and I hope that there will not be any issue.

I am attaching file with some photomicrograph of my research to check the staining of tissue by above mentioned method.

With Regards

Dr. Mahendra

you can fix tissue after you mount it on positively charged slides. This works and helps to have reasonably good H&Es