I tried to spot cardiolipin solution in chloroform on nitrocellulose membrane (Bio-Rad, 0.45 um pore size) to detect anti-cardiolipin antibodies in human serum. (this method of antigen immobilization on nitrocellulose is described in several papers, e.g. http://www.ncbi.nlm.nih.gov/pubmed/3512434). I spotted antigen on nitrocellulose strips and in the process of drying discs twisted. Authors used nitrocellulose with smaller pore size - 0.1 um and they didn't say anything about special membrane treatment before antigen adsorption. Can anybody explain this or give me advice about appropriate solvent for cardiolipin? I also tried butanol, ethanol, methanol and result always the same.
didi you find the solution? I also found the same problem, the organic solution deform the membrane quite a lot.
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