Pavel Khramtsov

3 Questions 14 Answers 0 Followers

Questions related from Pavel Khramtsov

Is it possible to calculate the ratio of hairpin/self-dimer ssDNA structures in solution?

I work with ssDNA aptamer. This aptamer binds target in hairpin conformation. I suppose that formation of undiserable self-dimer structures is possible during preliminary heating/cooling of...

01 December 2017 3,448 0 View

Do IgG bound to cardiolipin non-specifically or not?

Last week we adsorbed cardiolipin on polysterene microplates and nitrocellulose. We tested sera from healthy people and VDRL-positive sera. Sera from healthy people produced as a high signal as...

26 February 2015 8,331 7 View

Is it possible to avoid the nitrocellulose deformation when spotting lipid antigens in the chloroform?

I tried to spot cardiolipin solution in chloroform on nitrocellulose membrane (Bio-Rad, 0.45 um pore size) to detect anti-cardiolipin antibodies in human serum. (this method of antigen...

19 September 2014 908 1 View