Is it possible that Pacific blue can quench by protein like Lactate dehydrogenase?

More Himali Somaweera's questions See All

I am facing difficulties in opening a time series recording file in ImageJ. an error message with insufficient memory comes up. Any solutions?

The available memory keeps changing even if no file is open. The files that I want to open are large files of around 7-10 GB

22 July 2023 8,228 1 View

Are there any works discussing the stability of EKF with unknown inputs?

For the discrete-time Extended Kalman filter (EKF), a stochastic stability analysis is discussed by Reif et al. in their paper "Stochastic stability of the discrete-time extended Kalman filter"...

19 June 2021 2,969 3 View

Shall we apply decision tree classification directly on image?

Hi everyone, I wanna know how decision tree classifier can work on images. Shall I use HoG feature vectors directly as an input to decision tree classifier?

22 December 2020 5,268 6 View

Can any one say, Is it possible to implement ZSI in practically? Is there any manufacture who produce Z source inverters for solar PV applications?

I need to know whether industry are using advantages of ZSI and have it proven its advantages over buck boost converters.

24 June 2018 8,392 5 View

Calculate separation efficiency or resolution in isotachphoresis?

I have done isotachphoresis separation of a dye and need to calculate separation efficiency. it gives two bands. But there is no record for electrophoretic mobility of the dye. How you do this?

27 December 2017 8,011 2 View

I am looking for a narrowed down research problem/gap as a Research Topic in PROJECT MANAGEMENT as a part of my Master Thesis.

Please suggest a good area with practical problems that can be researched and useful to the industry. The issue is that I have just started MSc Business Project Management and have no deep idea...

01 January 1970 4,223 7 View

Conjugation of PEG-Amine to an Amino Acid Using EDC?

I am attempting to conjugate PEG to an amino acid at the C-terminus, for the purposes of producing nanoparticles. I have been told that PEG modified with amine groups can be used for this purpose,...

31 July 2024 2,033 1 View

EDC carbodiimide and NHS easter 's half life among different pH?

It has been long known that EDC carbodiimide and NHS easter's half life agains hydrolysis are highly related to pH. However, very surprisingly, not yet found a paper giving a table/chart of their...

25 July 2024 8,738 0 View

What does the difference \lambda_j - \lambda_{j+1} represent in relation to the conjugate partition of \lambda?

For a partition of an integer \lambda, what does the difference \lambda_j - \lambda_{j+1} represent in relation to the conjugate partition, , with \lambda_j being the j-th element of \lambda?

23 July 2024 8,008 1 View

Is it possible to conjugate an NHS ester to the N-terminus of a peptide ON RESIN?

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18 July 2024 4,075 3 View

Does anybody have Anti-Claudin8 and Anti-Claudin2 Primary conjugated Ab (Target Species: Human)?

I need Anti-Claudin8 Primary and Anti-Claudin2 conjugated Ab for my immunofluorescence experiment. If anybody has worked on it. Please share the accession number/details.

14 July 2024 485 0 View

Would it be possible to determine how many Cys I have based on Ellman's assay?

I want to predict the DAR ratio based on the number of cys available for conjugation to Cys residue. I use Ellman's assay to predict the concentration of Cys after TCEP reduction. I gain a...

02 July 2024 612 6 View

Conjugate Pad absorption suddenly changed?

Hello, I am doing some testing on lateral flow devices/materials and have run into a slight issue where I do not understand what the cause may be, but I have some ideas. General Experimental...

01 July 2024 7,960 3 View

Does anyone know what to do if Horiba ABX pentra 400 won't run positive and negative controls for LDH?

I am trying to run LDH assay on my samples using Hroiba ABX pentra 400. Multical has worked but negative and pos control are showing an error message saying no control in memory (image attached)....

18 June 2024 6,514 0 View

What is best stabilizing buffer for conjogation of quantum dots to antibodies?

We are researching the conjugation of various antibodies to quantum dot microspheres and europium microspheres. The sustainability results have been unsatisfactory. Which stabilizing buffers do...

25 May 2024 7,663 3 View

Q1: How can I improve hybridization and the process of hybridization?

Procedure: Streptavidin-magnetic bead suspension (50 µL) was transferred into a vial. The beads were washed twice by the binding/washing buffer and then re-suspended in 100µL of the buffer. A...

16 May 2024 893 0 View

Steingrimur Stefansson

Hi Himali, can you elaborate? Are you saying that fluorescence intensity is inversely proportional to the concentration of LDH protein concentration that is labeled?

Maksim Fomin

Hi Himali, I think the problem could be that NADH (cofactor of LDH) absorbs light in the same range as Pacific blue. Which wavelength do you use for excitation? Also fluorescence of Pacific blue depends on pH of your solution.