I exposed a green alga (pseudokirchneriella subcapitata) to copper and the fluorescent signal I am getting is way lower than that in the control, could it be due to the presence of the metal?
Hello, I dont know if you want to see DNA but maybe it can be due to the high affinity of metals for DNA blocking DAPI binding. Maybe this paper can help you http://www.actabp.pl/pdf/1_2015/7.pdf
I think it is OK, we did CLICK chemistry which contains Cu2+ and then perform the DAPI staining
Hello ,
your question is very obscure and must be reformulated. Why?
- Are you using copper metal or copper salts. if you use copper salts, what is your counter-anion (Chloride , sulphate...) and in what for a medium ? What a salt concentration are you using.
What a kind of fluo-spectrometer are you using? have you an alga suspension in your cuvettes?
JRG
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