I need to do a qRT PCR but there are not available filter tips?

Due to the COVID situation it is nearly impossible to find filter tips. Is it possible to do it without filter tips by making sure to clean the pipette with RNAse zap before use and working...

17 January 2021 8,839 3 View

Should I move forward on a study if I find there is a weak correlation, but not statistically significant?

I'm conducting a correlational study (Spearman rho) and there are no statistically significant relationships between the variables with a sample size of 37 (4 independent variables/1 dependent...

04 January 2021 5,976 5 View

Time to prepare samples for RNA-seq?

Hello, I am analysing the cost benefit of doing: RNA extraction and library preparations myself Sending RNA samples for sequencing Sending the entire sample for extraction and RNA preparation...

26 November 2020 2,203 2 View

How to analyze Lipid extraction (QDa HPLC) data?

Dear colleagues, I've been working on some lipid extraction analysis with QDa HPLC. I start with some biological samples that are spiked with a different final concentrations of a lipid mix...

14 March 2020 919 4 View

NCI-plot wfn file failure?

Hello, My name is Margarita Bulatova and I am a Ph.D. student from the University of Jyvaskyla, Finland. I hope that there is someone who can help me with my NCI calculations problem. I have...

24 January 2020 7,742 3 View

Doed anyone have a protocol for comet assay in chlamydomonas?

Hi everyone! I am looking for an efficient comey assay protocol in chlamydomonas reinhardtii if you have some suggestions let me know thanks a lot!

15 January 2020 698 3 View

Why do we test the water swelling behavior of a material which is tested for its suitability for bone tissue engineering?

I understand what water swelling behavior means, but how is that related with a materials properties to assist in bone regeneration ?

13 April 2019 4,549 4 View

Commercially available Arc/Arg3.1 antibodies for rat tissue?

Dear all, we are currently looking for a reliable anti-arc/arg3.1 antibodies which will be working in rat organotypic hippocampal slices. Has anyone already tried and know a good ones? We will be...

17 October 2018 9,794 2 View

How destruct the electrochemical activity of the polyaniline modified electrochemical (bio)sensors?

Regarding the polyaniline-modified electrochemical (bio)sensors: I suppose that we have to provoke a loss of the electrochemical activity of the polymer before the electrode application for the...

21 August 2018 2,129 2 View

Are there any organisms that benefit from air pollution?

In marine ecosystems, water pollution may give rise to the proliferation of algae and bacteria through eutrophicaton. Is there an organism that benefits from air pollution from vehicles and factories?

11 May 2017 7,384 7 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What test should i use in comparing the correlation between categorical and continuous variables?

Dear all, I have the variable (Surgery duration) represented by values in minutes and the variable (preoperative neuropraxia cases) represented as (Yes/No) values. I want to compare these two...

03 March 2021 5,427 5 View

Logistical regression using SPSS for a dichotomous dependent variable, with an independent variable of 1, 2, 5 and 10 seconds?

I have an experiment with 28 participants The independent variable is the times 1, 2, 5, and 10 used as the cross hairs during a task These 4 times were tested using 4 different blocks, each...

03 March 2021 9,692 2 View

How to find the mean, SD and CI in SPSS?

Hi, I hope that someone can help me with this task: I have 5 groups of participants which are numbered in SPSS from 1 to 5 (according to their group number) and are all under the variable group....

03 March 2021 506 3 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

How to code open-ended questions in SPSS?

Hello all, In SPSS I am going to code 2 open-ended questions. I have already read all the answers and I made a list of the most important categories to which I can code the answers. This question...

02 March 2021 1,757 4 View

The procedure compute?

02 March 2021 5,204 3 View