Statistics Question: Meta-analysis of factorial trials?

Hi all, is it possible to do a meta-analysis of randomized controlled trials with factorial design? I would appreciate some guidance/referral to a statistical resource or guide on this. Many thanks.

17 January 2021 4,090 3 View

Should I move forward on a study if I find there is a weak correlation, but not statistically significant?

I'm conducting a correlational study (Spearman rho) and there are no statistically significant relationships between the variables with a sample size of 37 (4 independent variables/1 dependent...

04 January 2021 5,976 5 View

Time to prepare samples for RNA-seq?

Hello, I am analysing the cost benefit of doing: RNA extraction and library preparations myself Sending RNA samples for sequencing Sending the entire sample for extraction and RNA preparation...

26 November 2020 2,203 2 View

What plasmid do you recommend to cloning promoter sequence from cassava?

13 July 2020 3,435 2 View

Can base pairs be lost from promoters during cloning DNA?

Hi everyone? I'm doing my thesis about Promoter regions in some jasmonate genes on cassava (M. esculenta). I have any questions about the DNA cloninig... Is possible lose some pair bases (bp) of...

25 April 2020 7,258 3 View

Is it better to use base e (LN) or base 10 (LOG10) when making estimates of body mass from linear measurements?

For linear regressions between linear measurements and body mass, which transformation is better to use? base e logarithm (LN)? or base 10 logaritm (LOG10). Apparently they do not make such a for...

08 April 2020 5,201 12 View

SPSS 26 (MacOS Catalina) R extension?

Hi! I am trying to install R extension on my SPSS 26 for Mac. I already downloaded R (version 3.6, as previous versions are not supported by Catalina). I looked for the R36 extension on the...

17 March 2020 174 5 View

How to analyze Lipid extraction (QDa HPLC) data?

Dear colleagues, I've been working on some lipid extraction analysis with QDa HPLC. I start with some biological samples that are spiked with a different final concentrations of a lipid mix...

14 March 2020 919 4 View

In the R package geomorph, how do I interpret the matrix of PC loadings as returned by $rotation?

I have exactly the same question as the person who is asking in this...

01 March 2020 7,298 2 View

NCI-plot wfn file failure?

Hello, My name is Margarita Bulatova and I am a Ph.D. student from the University of Jyvaskyla, Finland. I hope that there is someone who can help me with my NCI calculations problem. I have...

24 January 2020 7,742 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

How to calculate Total Flavonoid?

I analyzed flavonoid total spectrophotometry, the procedure as below: 2 ml supernatan added 5 ml dd.H2O, then added 0,15 ml NaNO2 5%, 5 minutes later added 0,15 ml AlCl3 10%, After 6 minutes,...

01 March 2021 6,722 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

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Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View