I'd like to do a lenti-virus transduction as soon as possible. Is it OK to thaw cells , directly plate them into 6-well plate, and after overnight, do the transduction?
As long as the cells are viable after the overnight culturing following thawing, they can be transduced. The key for a successful transduction is the density of cells and the mOI (tittering virus prep before the transduction). Adjust the number of cells plated to a confluency of 30-50% upon transduction.
I'm not sure that's gonna be a good way, since the cells that were storaged need recovery time. Therefore you'd better do the cell seeding first, and then right after you get the cells in proper confluency, you can subculture it into 6-well plate and do the transduction process. I think that's gonna be better even will take longer time.
It is not recommended to undergo experiments on newly thawed cells as thawed cells need at least 2 subcultures for recovery.
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