It is generally not recommended to store a protein A chromatography column in 20% ethanol without NaCl as this may damage the resin.
Protein A is commonly used for the purification of antibodies, and the Protein A resin is typically composed of cross-linked agarose beads that are coated with recombinant Protein A. The binding of the antibodies to Protein A is mediated by interactions between the Fc region of the antibody and the Protein A.
The presence of NaCl in the storage buffer is important for maintaining the stability of the Protein A resin. The salt helps to stabilize the Protein A on the resin, preventing denaturation or aggregation, which could lead to loss of binding capacity. Ethanol can also affect the stability of the Protein A by disrupting the tertiary structure of the protein, which could also lead to loss of binding capacity.
Therefore, it is generally recommended to store protein A chromatography columns in a buffer containing 20% ethanol and 0.5 M NaCl, or in a buffer containing 20% ethanol and 20 mM Tris-HCl (pH 7.5) without NaCl. This can help to maintain the stability of the Protein A resin during storage.
It is important to follow the manufacturer's recommendations for storage conditions for the specific Protein A resin that you are using to ensure optimal performance and longevity of the column.
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