Pegylation is a well established procedure. But is it important to optimize pegylation using a different molecular weight PEG at different concentrations to get the maximum coverage for a particular nanoparticle surface? Or is choosing a PEG (say 2000 Da) based on available literature sufficient?
The coverage of the nanoparticle surface depends of the nunber of functional grups that you have available to link the PEG. For instance you can use XPS to determine the atomic percentage (at.%) of the functional groups.
The best answer is: yes. The easiest answer is: try to do what they say in the literature, and see what happens. First of all, PEG-2000 is not 2000 Da. Secondly, coverage depends both on the dispersion energy (a geometric term referring to how tightly packed your PEGs are on the surface) and any local energy maxima (for instance the particle has a hydrophobic surface and is in water, and the "neutral" PEG localizes to the interface). PEG is relatively easy to work with, and you will get something no matter what you do. But knowing what you have when you are done is another issue.
i have a question rather : how can you link Peg diamine 2000 on a nanoparticle eg gold ? does anyone has a protocol?
PEG is available in wm 200, 500, 1000, 2000, 5000… etc. Literatures suggested that conjugating peg 2000 in drug; polymers considerably alter the physiochemical and pharmacokinetic properties of the molecule. So, after attaching peg 2000 u achieved u r goal not necessary to think about attaching other mw of the PEG. If u not achieved the goal then thing about other mw.
yes, I would do so, literature data can save time on trying too many, but you will need to optimize the protocol
Literature is good if the type of nanoparticle that you are working with has been attempted previously, or if the type of nanoparticle is closely related in structure or composition to nanoparticles with known procedures. The covarage usually follows an observable pattern, attempting a few options should start to give you an idea of what a specific PEG would do to your given particle, this should at least narrow the field down.
@Badri: You should be able to couple the PEG to the nanoparticle with the formation of a simple peptide bond.
The variables that you will need to focus on for optimization and what ranges you should examine (i.e., how much time and effort you're going to want to put in to this), are probably going to depend on (1) the application you have in mind for the particles, (2) the means of delivery (systemic, local, ex vivo) and their buffer, (3) the payload molecule(s), and the (4) the components of the nanoparticles themselves. For some applications and payload-polymer combinations, you can probably get away with minimal optimization while others may require more extensive studies. Just some things to think about as you survey the literature. Good luck! :)
You can use PEG 2000 and protocol in the literature if your nanoparticle has the same physicochemical properties as mentioned in the literature, check the physicochemical and pharmacokinetic properties of the pegilated nanoparticle. If you achieve your goal, you are not necessary to think about the optimization. If you do not achieve the goal then thing about the optimization.
Yes each case is individual. YOU can read these publications and some others from mine profile:
Article Polymerization Model for Hydrogen Peroxide Initiated Synthes...
This company has a lot of useful pegylation protocols and references fr the use of PEG derivatives and reagents on their website. You may find something useful.
http://www.creativepegworks.com/pegylation_literature.php
http://www.creativepegworks.com/pegylation_literature.php
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