How to do data analysis of a split plot design experiment with an extra control treatment outside the design but in the same field?

In an split plot experiment, 4 methods of Nitrogen(N) application was assigned in main plot and 3 doses of N in subplots and replicated thrice. Along with it, in the same field, No N control...

20 September 2020 9,944 2 View

Availability of Somatic Mutation Gene Panel in Breast Cancer?

Is there any somatic mutation gene panel for detecting breast cancer? I already know there are Germline mutation gene panel for Breast Cancer

09 July 2017 5,361 1 View

Can anyone help with Ansys 14.1 transient full mode analysis?

06 July 2015 9,580 3 View

Why core shell quantum dots is not dissolving in water or ethanol?

17 February 2015 4,963 3 View

What is the role of copper in anaerobic metabolism?

I want to know the role of copper in anaerobic metabolism and also I like to know the interaction between the nanoparticle and bacteria and how nanoparticle inhibit / enhance bacterial growth.

14 May 2014 9,429 2 View

Can anyone help with this Gaussian error message- "write error in NtrEx1"?

An error message " write error in NtrEx1" is showing when I was doing optimization and frequency calculation in Gaussian 09. Can anyone help me?

04 May 2014 8,930 5 View

How to dissolve Magnetite Nanoparticles?

I have synthesized Magnetite Nanoparticles using Cyanobacterial Polysaccharide crystals and as the nanoparticles are not dissolved, I am not able to proceed with my work . Can any one help me in...

24 March 2014 154 14 View

Which cell line can be used for in vitro glucose uptake activity?

C2C12, 3T3-L1, L-6 and HepG2: among these, which cell line will be ideal for in vitro glucose uptake activity?

09 February 2014 806 19 View

Where to purchase a cellometer?

Our lab is planning to purchase a cell counter for routine cell culture application, and we would appreciate any suggestions where to buy it.

15 November 2013 3,696 3 View

Is there any special protocol for RNA extraction from adipose tissue?

I use a commercial kit for RNA extraction, however I am not sure if it is suitable for adipose tissue. However, due to a high content of triglycerides do I need to have an extra step or something...

14 December 2012 6,546 6 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View