28 December 2017 2 7K Report

I'm trying to dissolve sodium palmitate for cell culture media. I used regular BSA previously and dissolved the sodium palm with ethanol but that method didn't work; the sodium palmitate chunked up when I added it to the media.

Is it necessary that I use fatty acid free BSA? If so, why?

Also, if anyone has any protocols where I can prepare sodium palm for cell culture, please do share!

Thank you.

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