Hello everyone.I used commercially available a 12.5mM TAE (Mg2+) buffer to mix my DNA sample and took scanning electron microscope (SEM) images of DNA GG and TT mismatches. Since SEM operators and I don’t have any idea whether its DNA or something else your expertise will be highly appreciated. If anybody is working on it, please share your experiences. The ring-like structure represents the figure from the GG mismatch, and the subsequent one represents the TT mismatch.Thank you.
I can not ID the material based solely on your image. I’ve seen similar shaped “crystals” for many things. However, I recognize the pattern formed as a result of a drop of your liquid material that has dried out, leaving a “ring” of crystals; a common occurrence for many substances, when the solution is dried. Some form of MassSpect, or perhaps Ramon, or FTIR could be used to ID the chemical crystals.
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