Molecular Biology, Molecular Genetics, Bioinformatics
By using only protein sequence data (as opposed to DNA sequence) you are disregarding information about silent mutations, that may be significant in reconstructing the tree.
I would like to know the answer and the solution procedure of this problem.
31 December 2019 5,506 3 View
The molecular weight of my protein sequence is 41 kDa. The SDS-PAGE result demonstrated its size 36 kDa. What are the reasons behind that?
01 February 2018 7,339 3 View
I would lie to develop some mutants.
01 February 2018 3,969 0 View
I would like to know the easy explanation for these tree points. I am happy if someone send me some articles or books about this.
01 February 2018 3,504 1 View
I got the excellent protein quantity after measuring the protein level through western blot analysis. Hence I am checking the corresponding mRNA in the qRT-PCR but failed to get proper mRNA...
10 November 2017 3,460 14 View
Gene , Molecular Biologist, Biostatics
10 November 2017 8,407 0 View
I would like to know the sodium sulfide actual state level in the solution. Biochemistry, Chemistry, Polymer chemistry, Biophysics
05 June 2017 4,835 7 View
Molecular Biology, Molecular docking, Biophysics
03 April 2017 8,190 0 View
Enzymology, Molecular biology, Biophysics
02 March 2017 8,053 3 View
Enzyme kinetics analysis, Biochemistry, Molecular Biology, Biophysics
11 December 2016 4,164 0 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
kindly reply me. Thanking you in advance.
05 August 2024 7,727 4 View
Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...
03 August 2024 3,371 3 View
I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?
02 August 2024 3,143 4 View
Molecular docking software/ websites?
02 August 2024 8,704 7 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View