If 0.9 mg/mL of an enzyme catalyzes the conversion of 25 micromoles of substrate per unit, what will be the unit of enzyme activity?

I would like to know the answer and the solution procedure of this problem.

31 December 2019 5,348 3 View

Why is the predicted molecular weight of the protein not authenticated by the SDS-PAGE result?

The molecular weight of my protein sequence is 41 kDa. The SDS-PAGE result demonstrated its size 36 kDa. What are the reasons behind that?

01 February 2018 7,130 3 View

Anyone could send me a book or manual related to site directed mutagenesis study?

I would lie to develop some mutants.

01 February 2018 3,785 0 View

What is promoter, motif and domain?

I would like to know the easy explanation for these tree points. I am happy if someone send me some articles or books about this.

01 February 2018 3,358 1 View

What is the possible reason for not getting the proper mRNA expression level in qRT-PCR compared to the western blot protein data?

I got the excellent protein quantity after measuring the protein level through western blot analysis. Hence I am checking the corresponding mRNA in the qRT-PCR but failed to get proper mRNA...

10 November 2017 3,246 14 View

Please tell me any link address for user friendly free gene network analysis software?

Gene , Molecular Biologist, Biostatics

10 November 2017 8,246 0 View

Anyone could tell me the actual state of Na2S in water?

I would like to know the sodium sulfide actual state level in the solution. Biochemistry, Chemistry, Polymer chemistry, Biophysics

05 June 2017 4,669 7 View

Any one could send me paper related Intra fragment interaction energy calculation between substrate and enzyme?

Molecular Biology, Molecular docking, Biophysics

03 April 2017 8,043 0 View

What should be the possible reasons that hinders substrate (ligand) to bind in the active site of receptor (protein)?

Enzymology, Molecular biology, Biophysics

02 March 2017 7,870 3 View

I would like to calculate the competitive and non competitive inhibition of enzyme. Could anyone send me a easy method for that?

11 December 2016 4,010 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View