I ordered a horseradish peroxidase-conjugated IgG antibody from Nordic Immunology (polyclonal goat atiserum to rhesus monkey- code GAMon/SC/PO) and it did not seem to dissolve in distilled water. The water seemed very clear and the reagent looked completed precipitated, not dissolving at all. I wonder whether this reagent is good for even though it doesn't seem to dissolve.
hi
"These insoluble particles may reduce your antibody reactivity and you need to use higher concentrations for your tests ."
Product Purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C.
good luck
Reza Hadavi thanks for your input. I had never worked with this antibody before and when I mixed with water it seemed like nothing was dissolved (cause there was a very high amount of insoluble particles). I will spin down accordingly and use it.
Best,
Sabrina
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