Yes, providing you know what the sympathetic response to excercise does to the blood flow to muscle and skin is in the forearm and hand. So far, the only way to measure perpheral and aortic blood pressure is invasively. 

I am the only investigator tha has compared aortic and radial blood pressure 

There is no harm in recording, because adult male or female are normal and can perform exercise. so what is wrong if you test the change in speed does affect the blood pressure like the change in speed does affect electrocardiography (ECG).

so you can measure in adult normal (with no cardiovascular problem)male/female 

It is mandate to check bp while stress test because Hypertensive  bp response is an indication  to stop the tmt as it risks intracranial  hemorrhage  and seondly fall in bp upto 20 mmhg during  stress indicates  a  positive  outcome. 

In research study, researcher did exercise with speed of 6 km/hour for male and 4.5 km/hour for female on tread-mill following Bruce protocol stage 2. So is it advisable or compulsory keep different speed for male and female?

No separate  protocol for male or female  is needed

Alfredo L Pauca MD. Wake Forest University School of Medicine

The conventional blood pressure measured by the cuff does not tell us whether the displayed systolic blood pressure is related to the flow or pressure velocity in the proximal aorta. These are completely different; the flow velocity related, P1  indicates how forcefully the LV is ejecting,while the 2nd, P2 is directly related to aortic stiffness and and also shows whether such stiffness is reversible (due to increased vascular tone, etc) or degenerative. The cuff blood pressure only shows the higher of the two, which is not very helpful if you want helpful findings. However, comparing radial and aortic PWs obtained invasively we found that when the radial and aortic systolic pressures were equivalent, both systolic pressures were represented by P2 in 9 of 51 patients (Pauca et al. Chest. 1992;102: 1193-98). This meant that the aortic SP, related to aortic stiffness, could be found in the radial PW. We tested this hypothesis in 45 patients, finding that P2 was equivalent in radial and aortic PWs in all patients (Pauca et al. Br J Anaesth. 2004;92:651-57) This finding has been explained by wave intensity analysis (Hughes AD et al. Hypertension. 2008; 51:e45-46). At present we have a method to identify P2 in radial PWs obtained invasively, which is as useful when these waves are obtained by tonometry. Our findings together with objective findings by researchers from the Imperial College UK and Calgary University, Canada, make it it possible (by inspection of the PW)  to tell whether a PW belongs to a young normotensive, a middle-aged, or an older  subject. Hypertension produces very expressive PW changes; it seems that it would allow us to tell whether a sympathetic or vasoactive treatment has an effect on P1 or P2. It seems that one day it should be possible to read the radial PW very much like we read an EKG.

I am not suggesting that you should use this methodology for your study, but to encourage exploration of the future. 

Of course it it right to measure the blood pressure because stress is going to produce a change and you might find that some individuals who might be harmed. From that point of view you have the duty to check it. However, your results would be more meaningful if you can tell from the results why has the blood pressure has changed. 

No, I am not trying to say that; I said that in order to get up to date information we should know what systolic pressure we are measuring. One is the augmented systolic pressure and the other is the central or aortic systolic pressure. The first is frequently seen in young males because their upper arm arteries are well developed; not so in females because their arms are not as developed as in men, even if they are weightlifters . The other type of systolic hypertension is central (or aortic); this is really pathological and  is seen in middle-aged and older hypertensives. It is due to aortic stiffening and needs medical attention. Unfortunately the blood pressure measured on the arm by a cuff will not tell you which SP you are measuring. Do not worry, soon we should have a simple and reliable method. It was proposed by us and has been confirmed by serious invasive and noninvasive studies.