Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Schrodinger Desmond runs fine on one GPU, but crashes on other GPU?

Hi, We are having issues using Desmond on our workstation. Simulations run fine on one of our gpus ("Gpu0") but crash consistently on the other gpu ("gpu1" - which is also set up as the display...

01 February 2021 5,866 3 View

Using LINK11 to Apply force in ANSYS APDL?

I'm trying to use LINK11 to apply a force as if it was a cable pulling a structure twowards a point in ANSYS APDL. When I solve the model, the deformation of the overall structure is far too...

30 January 2021 7,919 1 View

How does climate change affect birds (shorebirds)?

I m recently investigating the impact of climate change on shorebird population trends, I would like to know what is the impact of fluctuations in variables such as sea surface temperature, global...

10 November 2020 9,293 15 View

Can I use the EPIC-Oxford FFQ for free?

Designing my PhD project and need to use an FFQ, but we don't have any funding for this part of the project.

09 November 2020 4,541 1 View

Is there a survey tool (similar to REDCap) through which we can send an anonymous survey and restrict respondents to one survey each?

I am working on a research survey where I need to send it out to a group of users and track in (anonymous) responses. I need to restrict users to completing the survey only once. I do not think...

06 August 2020 918 2 View

Western Blot Transfer Band Smearing?

Anyone have any insight? It's becoming a recurring issue. I make fresh transfer buffer each time.

29 July 2020 9,331 9 View

How many users can i put in the same vsat broadband plan?

sorry if i wasn't able to explain very well for example, i am in a country that doesn't have satellite internet service, and would like to be the first provider for this service. lets say i have a...

05 July 2020 4,637 1 View

Failed gateway cloning?

Hi everyone, I am having a few doubts about gateway cloning. We are using gateway cloning for protein expression using pDEST15 as our destination vector. I attached a picture of the pDEST15...

10 March 2020 7,472 2 View

Inrush control when Transformers are Energized at Solar Renewable is best achieved how?

Increasing use of solar renewable resources or farms on weak remote locations are causing significant power quality and voltage notch issues with connecting utilities. High penetration of these...

17 August 2019 7,097 2 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View

Can polymer concentrations be calculated based on the number average molecular weight?

Hello, I spin-cast two different polymers onto wafers and measure their height as a function of initial concentration. For polymer A, I have the weight-average molecular weight (Mw) as well as...

28 February 2021 7,882 2 View

How can I publish a manuscript in free or low cost journal with good H-Index?

Dear all I am going to publish my manuscript which is from my thesis in a good H-Index journal but I found them very expensive(I don't have financial support) based on the exchange currency of my...

28 February 2021 7,800 6 View

The Shannon's index is inversely proportional to Simpson's index (1-D)?

I'm having a problem with my bloxplot with Shannon's and Simpson's indexs. The value of Shannon for my Community 1 indicating higher diversity (in relation of the Community 2), but have lower...

26 February 2021 3,033 9 View

Fractional impervious surface (FIS) map ?

Need Help Does anyone Know how to make a Fractional impervious surface (FIS) map from NDVI map, in ArcGIS environment? Thanks in advance

24 February 2021 8,455 2 View

What is the most important parameter of a research work, the impact factor or the h index?

The impact factor is a measure of the importance of a journal based on the number of citations received in a year. ... Articles published in a journal with a high impact factor do not all receive...

24 February 2021 7,481 2 View

How to dissolve cross-linked Amberlite IRC120 H, hydrogen form resin?

Hello all, I have used Amberlite IRC120 H, hydrogen form acidic cation-exchange resin for the ion-exchange of waterglass [sodium silicate solution -- 40% Sodium silicate (mol ratio > 3.2)...

21 February 2021 5,614 3 View

How to calculate refractive index (n and kvalue) against energy (in eV) data for alloyed nanomaterials through Independent Phase Approximation method?

Suggest me the method or some other software to calculate the above said data for nanoparticles.

21 February 2021 3,350 7 View

How to get/count refractive index in gasses?

20 February 2021 8,202 6 View

How to realize refractive index values of 1 to 1.1?

Hello researchers! While studying the literature on refractive index sensors, most of the articles simulate their devices with a refractive index ranging from 1 to 1.1, with small steps in...

17 February 2021 1,578 4 View