Does anyone use FACS to sort adult astrocyte from mouse brain?

What is the specific marker of adult astrocyte? I am trying to sort astrocyte from adult mouse brain by flow cytometry. I gated cd11b- cells and stain them with GFAP. But it is weird. My cd11b +...

01 February 2015 3,232 10 View

How do I assess neuron activation in vitro?

I am going to do some neuron and microglia coculture and i need positive control for neuron activation. I tried to test the P-ERK by western blot after AngII treatment. However, it seems no...

11 December 2014 8,887 3 View

Is it possible to measurement Ca2+ concentration in neurons using a microplate reader?

I want to use fluo-3 to detect the intracellular Ca2+ and I used spectramax M5. Can I use serum-free medium as the buffer?

11 December 2014 4,120 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

How to plot the probability paper of Gumbel distribution using R programing?

In R-studio, there are many commands of Gumbel package. Arguments are also different. I`m asking about the alpha parameter of the Copula which must be greater than 1. If this is the one used to...

25 February 2021 5,229 3 View

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23 February 2021 4,014 3 View

Are there methods for automatically mapping floodplains, fans and terraces?

I want to map these river geoforms with as little subjectivity as possible. I have a high resolution DEM generated by drone data.

22 February 2021 7,800 3 View

Do I need to introduce HFD to ob/ob mice to obtain a T2DM model?

I am planning to work on T2 diabetic animals and check the efficacy of probiotics. My initial plan is to fed B6 animals with HFD for a while. But now, I have the chance to work on ob/ob mice. My...

18 February 2021 6,896 3 View

How to perform EEG classification of Alzheimer and control groups?

I am doing a project on classification of EEG signals of Alzheimer's disease and control groups. Previously, I extracted the alpha power of the EEG signals and found out the strengths. I want a...

18 February 2021 8,502 2 View

What are the options for global scale hydrology DEM?

Hello, I am working on a global scale hydrology GIS project delineating global watersheds and doing some data analysis. I have in the past used hydro1K from the USGS that cover the entire world....

18 February 2021 5,109 5 View

Why is there violent fluctuation of ThT signal in Amyloid beta aggregation?

Dear all, I am currently working on amyloid beta aggregation. In ThT monitered aggregation assay, I have already obeserved an increase in ThT signal, indicating the aggregation of amyloid beta....

15 February 2021 4,852 2 View

Can I use a categorical covariate in a Generalized Linear Model in SPSS v.26?

Hello everyone, hope you are well. I am planning to use a Generalized Linear Model in SPSS. I would like to add gender to the model as a covariate, however from what I have read, only continuous...

14 February 2021 8,937 4 View