You Li

4 Questions 6 Answers 0 Followers

Questions related from You Li

Is intracranial injection of beta amyloid acceptale as a Alzheimer's disease model?

I am trying to evaluate my drug efficacy of clearance of beta amyloid in vivo. but I am not sure whether it is acceptable to inject the beta amyloid intracranially as a AD model?

08 August 2018 3,169 0 View

Does anyone use FACS to sort adult astrocyte from mouse brain?

What is the specific marker of adult astrocyte? I am trying to sort astrocyte from adult mouse brain by flow cytometry. I gated cd11b- cells and stain them with GFAP. But it is weird. My cd11b +...

02 February 2015 3,327 10 View

How do I assess neuron activation in vitro?

I am going to do some neuron and microglia coculture and i need positive control for neuron activation. I tried to test the P-ERK by western blot after AngII treatment. However, it seems no...

12 December 2014 9,008 3 View

Is it possible to measurement Ca2+ concentration in neurons using a microplate reader?

I want to use fluo-3 to detect the intracellular Ca2+ and I used spectramax M5. Can I use serum-free medium as the buffer?

12 December 2014 4,219 3 View