Do mouse primary hepatocytes survive in transfection solution without serum ?

hello, i am planning to transfect mouse primary hepatocytes with miRNA in order to isolate microsomes to study the activity of my enzyme of interest. Therefore, I wondered if the primary...

07 August 2019 2,067 2 View

How to calculate amount of beta-glucuronidase in powder from 0.1 M sucrose?

Hello, how can I calculate the actual amount (in mg) of beta-glucuronidase from supplied white powder lyophilized from 0.1 M sucrose plus 1 mg sucrose per mg protein ? On the delivered vial it is...

09 October 2018 4,766 0 View

How to measure endogenous miRNA expression from 2 different body compartments ?

Hello RG community, My task is to measure the expression of several microRNAs from total RNA isolated from human body sera. I have a number of TIPS patient (suffering from liver cirrhosis) and...

07 August 2018 5,142 1 View

What is best reference gene when quatifying circulating miRNA from TIPS patient serum ?

Hi there, I received extracted whole RNA from TIPS patient serum and negative healthy controls. All samples contained spiked-in SV40 miRNA already as a reference gene. I performed miRNA reverse...

03 April 2018 3,960 5 View

Who could provide primers for quantification of retinoid X receptor alpha (RXRA) mRNA?

02 March 2018 1,051 5 View

How can I achieve to mutagenize a restriction site in a 6.5 kb plasmid?

Hi all, I tried to insert a restriction site in a 6.5 kb plasmid having only six endogenous restriction sites in the MCS. I already tried several primer setups but all failed. What could I...

10 November 2017 3,495 10 View

Is the number of clone picking to find integrated restriction site in plasmid equal to general process of insert screening ?

08 September 2017 8,176 4 View

How to exclude interactions of miRNA with the Luciferase reporter plasmid?

Hi all, does someone have experience with studying the interaction of microRNA (miRNA) and its target sequence cloned into a Luciferase Reporter vector? Actually using pGl3-Basic vector from...

07 August 2017 3,539 5 View

How to get luciferase reporter vector pMIR-REPORT?

07 August 2017 1,959 4 View

How can I use the Neubauer cell counting chamber?

08 September 2016 8,917 2 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Can NFL theorem be valid in infinite search space in ML?

NFL theorem is valid for algorithms training in fixed training set. However, the general characteristic of algorithms in expanded or open dataset has not been proved yet. Could you show your...

01 March 2021 1,189 3 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

Can water be extracted from Olivine?

I have always been interested in geology and wondered about how unique structures of materials behave in different circumstances. I stumbled upon Olivine, the magnesium iron silicate, and heard...

28 February 2021 7,330 4 View