Do mouse primary hepatocytes survive in transfection solution without serum ?

hello, i am planning to transfect mouse primary hepatocytes with miRNA in order to isolate microsomes to study the activity of my enzyme of interest. Therefore, I wondered if the primary...

07 August 2019 2,067 2 View

How to measure endogenous miRNA expression from 2 different body compartments ?

Hello RG community, My task is to measure the expression of several microRNAs from total RNA isolated from human body sera. I have a number of TIPS patient (suffering from liver cirrhosis) and...

07 August 2018 5,142 1 View

What is best reference gene when quatifying circulating miRNA from TIPS patient serum ?

Hi there, I received extracted whole RNA from TIPS patient serum and negative healthy controls. All samples contained spiked-in SV40 miRNA already as a reference gene. I performed miRNA reverse...

03 April 2018 3,960 5 View

Who could provide primers for quantification of retinoid X receptor alpha (RXRA) mRNA?

02 March 2018 1,051 5 View

Is glucuronide CDCA-24G soluble in water or in any other solvent?

Hi guys, I have a question about glucuronide CDCA-24G, maybe someone has already worked with it. I want to perform an experiment in hepatocytes which I have extracted from mouse livers. I do not...

01 February 2018 1,856 2 View

How can I achieve to mutagenize a restriction site in a 6.5 kb plasmid?

Hi all, I tried to insert a restriction site in a 6.5 kb plasmid having only six endogenous restriction sites in the MCS. I already tried several primer setups but all failed. What could I...

10 November 2017 3,495 10 View

Is the number of clone picking to find integrated restriction site in plasmid equal to general process of insert screening ?

08 September 2017 8,176 4 View

How to exclude interactions of miRNA with the Luciferase reporter plasmid?

Hi all, does someone have experience with studying the interaction of microRNA (miRNA) and its target sequence cloned into a Luciferase Reporter vector? Actually using pGl3-Basic vector from...

07 August 2017 3,539 5 View

How to get luciferase reporter vector pMIR-REPORT?

07 August 2017 1,959 4 View

How can I use the Neubauer cell counting chamber?

08 September 2016 8,917 2 View

What is the status of bread making and more bakery foods with the use of insect flour/powder?

Insects have been consumed by human diet since prehistoric times. At present, the idea of looking at insects as food has been getting traction worldwide, also in response to increasing food...

03 March 2021 6,223 5 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Have anyone checked before Cyclic voltammograms of intial ITO substrate and E-teck?

As a beginner in electrochemistry, I tried to obtain cyclic voltamograms of commercial anatase powder deposited on an ITO substrate or on Eteck. After several attempts, I noticed that my cyclic...

23 February 2021 3,175 3 View

How to reduce static electricity of powder after lyophilization?

I am working on synthesizing and then purifying peptides using the SPPS system. After purification of the peptide, the peptide powder is obtained using a freeze dryer. When measuring the powder,...

22 February 2021 10,099 5 View

What kind of steel moulds are used for hot pressing metal powders?

My main concern is that the metal powders should not weld to the mold surface and I should easily be able to eject out the pressed samples. I am looking for alternative for graphite moulds. The...

22 February 2021 2,965 4 View

How to Study a proton NMR of Graphene prepared by Electrochemical Exfoliation technique?

I performed a Proton NMR of graphene powder prepared by electrochemical exfoliation technique. It shows some peaks. But I can't understand it.

21 February 2021 8,947 3 View

Are ZnS nanoparticles moisture sensitive?

Hello Everyone, I tried to form ZnS nanoparticles using the hot injection method and was able to form the same (confirmed by XRD). When I centrifuged, after the reaction I got a kind of white...

17 February 2021 1,709 3 View

Pile-Raft connection?

Greetings, I was wondering what is the optimal way to connect the reinforcement at the top of the pile (the part that connects to the raft foundation): -The pile bars are left straight and...

16 February 2021 2,007 3 View

How to dissolve lyophilized silk fibroin?

Dear members of the community, I am trying to concentrate silk fibroin to 25 % (w/v). Nevertheless, i am struggling with the concentration step. I successfully achieve concentrations of 5-6 % of...

16 February 2021 1,808 1 View

How does a freeze dryer destruct your solid sample?

Several times I lost my sample during freeze drying. There was no solid or powder in the vial after freeze drying. I'm sure sample contained solute because i took its NMR spectrum. I covered the...

15 February 2021 6,749 2 View