Hello all,
I wondered whether the equation is right that we use in the lab for HepG2 cell counting with the Neubauer counting chamber.
We generelly use a sample volume between 40-50 µL of cells which is resuspended in the sample volume of Trypan blue (so 1:2 dilution). After counting 4 big squares, the equation Looks like this:
Cells counted x 2 (for Dilution) x 2500 x total volume = total cell number
I think this is correct but would appreciated if you could shortly look over this equation and give me a comment please.
Thanks in advance, Stefan
Hi Cornelius,
Thanks so much for responding quickly. You are absolutely right, it's so simple. I was confused by the different square sizes and different calculations. Wikipedia explains very well either, actually we count 64 of the green squares (according to your wiki link) but this gives an overall volume of 0.4 microliter as you have correctly indicated. Following the calculation, this gives the total cell count. Thanks for your help, appreciate!
I explained the method of neubar counting for spore of fungi in my publications. Please see them
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