Minimal protein concentration from BCA can be detected using western blot?

hello, i extracted protein from PBMC's and after applying BCA assay on 2 samples the protein concentration was 400 ug/ml which means 0.4 ug/ul so does western blot work with these quantities? any...

24 December 2020 6,521 3 View

Why does the value of displacement applied results in different Load-deflection curve behavior?

Hello, I am trying to model a four point bending for RCC beam using abaqus CDP model. I used displacement control type. The issue is whenever I change the value of the applied displacement, it...

25 September 2020 4,395 2 View

What is the fastest and most efficient method to wash acidic activated carbon?

The carbon was activated using phososhoric acid.

03 March 2020 5,256 4 View

What is the best preservation of RNA extract?

I need to know the best way to preserve RNA extract from tissue for a long period.

27 October 2017 4,716 9 View

Dear researchers, By any chance do you know any center that has clinical trial in stem cell therapy for type 1diabetes?

Case is a 4 year old kid, recently diagnosed with type I diabetes, seeking for new opportunities and novel treatments. Beside, parents banked his/her cord blood stem cells. I appreciate if you...

07 May 2016 7,210 4 View

Do you know any studies or books about artificial intelligence and supply chain management ?

18 December 2015 6,601 4 View

What are the disadvantages of using Prestoblue assay to quantify the cell viability in post-ischemic model of cardiomyocytes?

21 February 2015 8,712 2 View

How can I avoid high mortality of rotenone-induced PD?

I used 1.5mg/kg dissolved in DMSO/PEG 1:1 and I have high mortality intraperitoneal injection.

30 October 2014 4,152 1 View

How can I estimate the sequence of the SiRNA that will be used?

I want to prevent the expression of 11 amino acid in the peptide chain using siRNA,

16 February 2014 5,441 2 View

If I want to calculate the MRCA for Bufo arabicus using RelTime, what calibrated taxa do I have to select?

And what is the divergence time I have to use? Thanks in advance.

03 February 2014 273 1 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

Gas sensor response and recovery time independant of flow rate and chamber volume?

I am trying to obtain the response and recovery times for my gas sensor device. It appears the the response/recovery times are primarily dependant on the flow rate of the gas I inject into the...

26 February 2021 1,511 3 View

Open Source IoT Simulators supported by Machine Learning?

Dear Research Gaters, Let me first add context to the question. I am looking to simulate the waste management system and resources of a city. The hardware devices I need to simulate include,...

21 February 2021 2,333 4 View

What type of pressure sensor are usually used in microfluidics?

My team is designing a dual channel microfluidic chip that runs air and water. I think we've decided on the NE1000 syringe pump for it's versatility and feedback control. We will be ordering the...

18 February 2021 9,928 2 View

Do I need to introduce HFD to ob/ob mice to obtain a T2DM model?

I am planning to work on T2 diabetic animals and check the efficacy of probiotics. My initial plan is to fed B6 animals with HFD for a while. But now, I have the chance to work on ob/ob mice. My...

18 February 2021 6,896 3 View

How to realize refractive index values of 1 to 1.1?

Hello researchers! While studying the literature on refractive index sensors, most of the articles simulate their devices with a refractive index ranging from 1 to 1.1, with small steps in...

17 February 2021 1,578 4 View

Fluo 3-AM in pancreatic beta-cells?

Dear all, I'm trying to understand if a molecule of my interest (capable of inducing glucose-stimulated insulin secretion) determines an increase in intracellular calcium levels in the presence of...

16 February 2021 453 2 View

What should a smart water system look like?

Our team is designing a smart water C&C dashboard for a smart city initiative. As we look into the current smart water system landscape, we couldn't reconcile to a definitive solution that...

16 February 2021 1,094 3 View

Receiving very less number of hits and events in acoustic emission tests?

I am doing a three point bend test with concrete specimen of dimension 280mm*90mm*90mm. I am putting 2 sensors near the notch with 3cms away from both the sides. the experiment ran for 20 mins but...

13 February 2021 2,990 1 View

How to increase coupling range (distance between reader and sensor coils) in LC resonance circuit?

Hello great researchers, I am working on a wireless pressure monitoring project where sensing part is actually an LC resonance sensor and I am using a planar coil as a reader to get wireless...

11 February 2021 4,148 5 View