I want to extract curcumin and, if it's not pure, I won't be able to use the spectrophotometer in order to prove the presence of curcumin in the extract, because the existence of other substances would affect the measurement.
You need a characterization method to know if you have your compound or not, even if ethanol was able to extract curcumin you need to prove it.
No, other compounds will be extracted. You can use silica gel column chromatography with a dichloromethane: ethanol gradient to purify the curcumin sufficiently for spectroscopic analysis.
See:
http://www.teledyneisco.com/en-uk/liquidChromatography/Chromatography%20Documents/Posters/Purification%20of%20Natural%20Products%20Using%20Mass%20Directed%20Fractionation%20Poster.pdf#search=curcumin
Dear Victor,
Which curcumin you need to extract...?
Curcumin extract containing many other compositions such as tetrahydro curcumin, bisdemethoxy curcumin, demethoxy curcumin etc. You should be very carefull while extracting the pure curcumin because ethanol dissolves all other compositions in it. The best option is, you can purify curcumin just by passing through silica column by using ethyl acetate and and petroleum ether as a mobile phase. finally you should confirm by NMR spectroscopy.
To answer the questions, yes, i want to use spectroscopy and a curcumin standard purity 95%+. I need all curcumins, but nothing else.
yes it is possible by column chromotography. on the basis of RF value its possible to idintify the required product spot. just for information regarding appearence, tetrahydrocurcumin is white powder, bisdemethoxycurcumin is quite similar to brownish red and curcumin is yellow colour. just try to know the RF of each.
See organic process research dev,20(9):1593-1602 (2016) for methods of purifying demethixy curcumin from crude extracts
Extraction of Curcumin was done by using solvents ethanol, hexane, acetone and HPLC method to determine the best solvent for Curcumin extraction. Boiled and steam cooked turmeric was used for solvent extraction to quantify the percentage of Curcumin retaining in turmeric. Also to know the variety suitable for Curcumin extraction and on that basis the quality of turmeric and its market value can be judged which will be helpful to farmers, businessmen, industrialist and consumers. Fresh rhizomes of four varieties were washed, boiled, steam cooked, dried, polished grinded and Curcumin was extracted by ethanol, acetone, hexane and HPLC method. The highest percentage of Curcumin was extracted from variety Pratibha as compared with other three varieties. It was concluded that Curcumin retained in steam cooked turmeric is higher than boiled turmeric for all varieties in all methods of Curcumin extraction. The Curcumin extracted by HPLC method (7.73%) is higher than other three methods. The ethanol is the best solvent for Curcumin extraction than acetone and hexane for all varieties of turmeric.
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