What is the mechanism that allows antibodies to pass the blood vessel wall and enter the bloodstream?

I want to know about the antibodies capability to pass through tissues, especially blood vessel wall tissue.

10 November 2018 5,050 1 View

Would an antibody be destroyed in a mixture of isopropyl alcohol?

Are there any articles related to how different substances would affect antibodies, like preservatives and such?

09 October 2018 1,728 0 View

Can antibodies reach the bloodstream through skin, if someone applies an ointment containing them?

What effect would they have if applied on a wound? Antibodies, from another organism, applied on a wound, by the means of ointments and the like.

09 October 2018 8,761 3 View

How would someone separate proteins from lipids in a mixture, as in to extract them separatedly?

How do I do that, if the proteins are temperature sensitive?

31 December 2017 9,268 5 View

What would be the less costly methods for extracting a plante-derived substance out of a solid mixture (powder)?

Asuming I've combined a few substances extracted from plants, in order to make a powder, how do I extract them back from the powder in order to check if they are in proper proportions in the mixture?

31 December 2017 7,210 1 View

Is ethanol enough to extract pure curcumin (I don't have acces to HPLC)?

I want to extract curcumin and, if it's not pure, I won't be able to use the spectrophotometer in order to prove the presence of curcumin in the extract, because the existence of other substances...

11 December 2017 7,867 10 View

What would be the best method to sterilise seringes made of low density polyethylene?

I need to put in them some gel that is going to be used in stomatology.

10 November 2017 1,286 3 View

Does ethilic alcohol lose effect against bacteria when you go to a concentration greater than 70%?

I want to know if ethilic alcohol of +70% concentration loses its killing effect on bacteria.

09 October 2017 5,246 5 View

Is lysozyme hidrochloride more efficient against bacteria than simple lysozyme?

no details needed

07 August 2017 3,230 3 View

How do you determine how many patients you need for a study in order for the results to have statistical valuey?

I have nothing to add, it's all in the question.

06 July 2017 2,616 2 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

How to calculate the crystallinity of cellulose film?

27 February 2021 2,753 4 View

Can two EC50 values with different hill slopes be compared?

Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...

23 February 2021 8,155 5 View

Could the "incoming radiation (net radiation)" values be negative?

Could the "incoming solar radiation" values be negative? What is the difference between "global solar radiation" and "incoming solar radiation"? Which one might be measured negative values?

21 February 2021 7,726 9 View

In methylene blue test Why is UV absorbance reading very low as soon adsorbent is added and then increasing before reaching equilibrium?

Hello, I'm working on removal of methylene blue. 01. in the dark test very first reading is taken 15 minutes after adding adsorbent to the dye solution and that UV absorbance reading is very...

19 February 2021 472 5 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

What is the best temperature to coat an ELISA plate?

Hello, I want to control the temperature of my ELISA plate during incubations to get consistent results. In general, what is the best temperature to coat the plate with antibody? I have been...

16 February 2021 2,377 7 View

What is the optimal method to prepare a controlled porosity osmotic pump (CPOP) tablet for scanning electron microscopy?

In order to assess the shape and size of pores formed by dissolving the pore former in the semipermeable membrane structure, SEM images are needed. Cellulose acetate was used in the coating...

15 February 2021 6,135 3 View

How to calculate ultraviolet dose in Gy or J/kg?

Currently I'm doing a literature review regarding the comparisons of different types of radiation such as uv, electron beam and gamma radiation. But I'm having a difficult time converting uv unit...

11 February 2021 7,642 3 View