Is epigenetic regulation in fungus heritable?

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If i want to test a particular compound/metabolite on my yeast strains ?

If i want to test a particular compound/metabolite on my yeast strains ( which have been transformed with the desired disease plasmid and shows the phenotype) . Then what is the method that I...

06 July 2018 3,182 0 View

Area under disease progress curve?

In plant pathology experiments generally disease severity measured in terms of grades of affected leaf area in relation to time is used for plotting the area under the disease progress...

05 June 2018 8,058 0 View

Which is better to suspend extracted DNA?

Which is a better one to suspend extracted DNA? TE buffer or ultrapure water?

05 June 2018 3,942 4 View

Long term storage of leaf tissue in CTAB buffer?

Can a leaf tissue be suspended in CTAB buffer until its DNA extraction? like for example if i go to a field and collect the leaf tissue and suspend the leaf tissues in CTAB in an eppendorf tube...

05 June 2018 1,717 1 View

CTAB DNA extraction method for insects?

IS CTAB buffer method of DNA extraction suitable for extracting insect DNA?

05 June 2018 8,074 2 View

Avoiding clumping of DNA ladder?

my 100 bp and 1kb ladder are not separating properly in gel inspite of providing the optimum agarose concentration, voltage and running time. the individual size bands dont spatially separate...

05 June 2018 1,179 0 View

Pre heating of CTAB buffer?

In what way does preheating of CTAB buffer prior to DNA extraction from leaf tissues improve the efficacy of DNA extraction or DNA recovery

05 June 2018 3,705 4 View

Preserving clones in glycerol stock?

I have cloned my virus in puc 18 vector. Right now Iam maintaining the clones by repeated subculture on lb medium amended with ampicillin. Is it possible to maintain the clones in glycerol...

04 May 2018 3,604 2 View

Increased manganese availability in flooded soils?

plants like paddy dont suffer from manganese deficiency since they are grown in flooded soils? what is the reason for the increased availability of manganese in flooded soils?

03 April 2018 1,618 3 View

Readymix of mastermix and long term storage?

Is it possible to make a readymix of PCR reaction cocktail involving components like water, dntps, taq polymerase etc. and keep it under long term storage for future use or should we use the mix...

03 April 2018 9,933 3 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

How to define an anisotropic material with asymmetric elastic compliance/stiffness matrix in ANSYS APDL?

I need to model an anisotropic material in which the Poisson's ratio ν_12 ≠ ν_21 and so on. Therefore, the elastic compliance matrix wouldn't be a symmetric one. In ANSYS APDL, for TB,ANEL...

09 August 2024 5,048 2 View

Request Python code?

Request Python code from this article : Gender equity of authorship in pulmonary medicine over the past decade. THANKS!

08 August 2024 6,242 2 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Why does everyone use vs code?

Visual Studio Code (VS Code) has become a popular choice among developers for several reasons: 1. **Free and Open Source**: VS Code is free to use and open source, making it accessible to...

07 August 2024 7,013 4 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

How can i do multivariate Time Series forecast using MLP, ANFIS and LSTM?

I need the python code to forecast what crop production will be in the next decade considering climate and crop production variables as seen in the attached.csv file.

05 August 2024 2,977 3 View

What is fitofthora instance of potatoes?

Now research fellow used it to prevent fungus in potato,may be potatoes gene effect.

02 August 2024 180 3 View