Dhanya Ramakrishna Iyer

9 Questions 6 Answers 0 Followers

Questions related from Dhanya Ramakrishna Iyer

Technique to study burst and pausing?

Hi, I am looking to study transcriptional burst and RNA Pol II pausing at the promoter of a gene. What are the best methods to study such events at a locus? Most of the papers I saw focus on high...

05 June 2023 9,266 0 View

Telomere length determination from human genomic DNA?

In order to assess the telomere length in human blood samples by qpcr, what is the best standard reference one can use for drawing the standard curve? I came across papers which use a pool of...

04 March 2023 2,305 7 View

Fluorescent dyes in ischemia models?

I am inducing ischemia by OGD in vitro. If I want to estimate mitochondrial membrane potential or look at ROS levels by means of fluorescent dyes, can I fix the cells and then stain for these? I...

18 January 2023 2,234 4 View

Is chromatin intact in tissues stored in RNAlater?

I have some mice tissues stored in RNAlater. I was wondering if it is possible to do a ChIP or chromatin accessibility assay? Thanks in advance!

13 January 2022 4,944 2 View

Effect Size from beta & pval?

Hi, I want to do a meta analysis from GWAS and publications. For the GWAS studies, I only have the beta value, p value and total number of samples while for the publications I have sufficient data...

02 December 2021 1,102 5 View

Database for SNPs modulating chromatin accessibility?

Is there a database which quantifies or gives an idea of how chromatin accessibility or binding of a regulatory factor (TF or histone modification) is modified as a result of SNPs in the promoter...

18 November 2021 9,693 4 View

Is it possible to assay Nitric oxide generation in frozen human plasma samples?

Hi, I wanted to measure nitric oxide levels in human plasma, mostly using the Griess reagent. Can I use samples which have been stored in -80? Also, these plasma samples are stored in EDTA tubes....

10 April 2021 8,275 0 View

Beta value calculation of absolute intracellular calcium concentrations?

Hi all, I am doing calcium imaging experiments in N2a cells and wanted to calculate the absolute calcium concentrations. Can someone help me out in figuring out how to calculate the fluorescence...

25 March 2020 8,087 1 View

How do I select the best primer which does not produce any non specific bands from those I have designed?

Hi! I am currently designing primers for the human UGT1A1 gene using the Primer3 software. However, when I BLAST the primers against the Human RefSeq database, I get significant matches with other...

11 October 2016 7,247 3 View