How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

293-hTRPV1 stable cell line ?

Hi, I have some trouble with my HEK293-hTRPV1 stable cell line. I made a 293-hTRPV1 stable cell line with Flp system from Thermofischer...

16 September 2020 5,243 2 View

How much solvent (ethanol) would I need to extract capsaicin (and other capsaicinoids) from siling haba?

Siling haba is a variation of Capsicum annuum var longum. The capsaicin extracted will be tested against aphids to test its insecticidal properties. Someone suggested that I should use 5ml/g or 2g...

12 February 2019 1,373 3 View

How much ethanol do I need in extracting capsaicin from red bell peppers?

The capsaicin recovered will be tested against aphids.

04 February 2019 5,811 5 View

How much ethanol do I need in extracting capsaicin from red bell peppers?

The capsaicin recovered will be tested against aphids.

01 February 2019 9,877 6 View

Can anyone provide me a simple method to determine level of capsaicin using HPLC method?

I am about to carry out HPLC method for quantitative determination of capsaicin in my chilies extract. Can anyone provide me a simple method? Thank you.

05 June 2018 3,866 3 View

Can anyone help me to understand the mechanism of capsaicin antibacterial activity?

how the capsaicin (the pepper extract) can be has antibacterial activity?

04 May 2018 2,235 4 View

Would any body give a calorimetric or spectrophotometric method of determination of capsaicin from chillil? I am grateful to him/her

04 May 2017 4,793 1 View

Can two divided cells get together to become one?

I have recently performed an experiment in which I treated primary mesothelial cell with 300 uM of capsaicin and I recorded their behavior for 40 hours in order to see if there would be any...

08 September 2016 2,127 10 View

Hello, does anybody have experience with measuring caspase-3 and glutamine-synthetase by ELISA test in liver tissue homogenate?

07 August 2016 6,119 3 View

Hello, does anybody have experience with measuring caspase-3 and glutamine-synthetase by ELISA test in liver tissue homogenate?

07 August 2016 8,441 3 View