Hi Gregory!
To my knowledge and experience it's enough to autoclave or UV sterilise for DNAse denauration. But for RNAse denaturation autoclaving is not enough. You might denaturate RNAse by using UV sterilisation for a long time!
Best Britta
Hi Gregory!
To my knowledge and experience it's enough to autoclave or UV sterilise for DNAse denauration. But for RNAse denaturation autoclaving is not enough. You might denaturate RNAse by using UV sterilisation for a long time!
Best Britta
DNAse enzymes are pretty labile, they can even be denatured by physical force such as vortexing. RNases are much more robust. As Britta says, autoclaving alone is not sufficient, you need to eg. treat with DEPC first. I'm not sure UV would be sufficient due to lack penetration. For example if you were trying to remove RNase from solution in a glass bottle, the glass will block UVB. This is all assuming UV will actually inactivate RNase in the first place!
As they say for DNAse enzymes are labile enough, but for RNAse you need to treat with some chemicals eg DEPc or RNAse inhibitors solutions.
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