I would like to transfect some mammalian cell lines that I am using for research on recombinant antibody production. In order to increase transfection efficiency I am thinking on using electroporation systems so I would like to ask for protocols, tricks and experience with that technique.
Hola Leticia.
En estos links encontrarás manuales y papers que te pueden orientar.
Saludos.
http://www.btxonline.com/eukaryote-cell-transfection/
https://online-shop.eppendorf.com/OC-en/Cell-Manipulation-44522/Electroporation-44523/Eppendorf-Eporator-PF-56096.HTML
http://biorad-ads.com/transfection_protocols/
Hello Leticia, you may check the Scientific Support of Lonza Bioresearch (which amaxa is part of).
Scientific Support Telephone: +49 221 99199 400, Fax: +49 221 99199 499, [email protected]
You also may find useful Information on the amaxa Nucleofector here: http://bio.lonza.com/resources/product-instructions/protocols/
All the best. Bodo
Muchas gracias Antonio Navarro and also thanks to Bodo Ortmann. The links you have provided will be really useful although I still look for someone who has the device so I could (maybe) see it's performance in person.
Thanks again!
Hi Leticia, you can get in touch with our partner Cultek in Spain, they can discuss a demonstration with a Nucleofector.
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