How to best measure room light with apps?

Hey! I am currently working with anxiety tests in animal models (light-dark box, elevated plus maze) and I am some trouble to check the room light (Lux). I have downloaded an app (Lux Light Meter)...

15 February 2021 3,830 3 View

Which statistical test when comparing 2 cell groups over time?

I need to compare the transport of a compound across 2 different cell lines (non-transfected vs. transfected) over time. My goal is to compare the mean transport between Cell 1 and Cell 2 at time...

08 July 2020 2,410 5 View

Protease activity assay?

Hello, I would like to get help! I want to measure the protease activity of pancrease powder. I prepared 10mg/ml stock solution from the pancrease powder and I used 100ul from that and I added...

22 June 2020 1,609 3 View

Does anyone has good references to "Organic x Inorganic CO2 in the atmosphere"? Volcanos x Burning fossil fuels?

I need more details and references about: the increase in carbon observed in the atmosphere is the organic origin CO2, not the inorganic origin CO2 released when a volcano erupts. Evaluation of...

19 March 2020 948 3 View

What is the most accepted probabilistic criterion considering frequency layers (fire) via distribution modeling?

fire index prediction

15 September 2019 3,112 2 View

Which Polymerase high fidelity is best to detect mutations (PCR followed by Sanger sequencing)??

I am detecting mutations in virus genome, using PCR for 1kb followed by Sanger sequencing. GoTaq (Promega) works greatlly, but PCR with Pfu Polymerase gives a small amount of amplicon. I followed...

12 March 2018 4,553 4 View

Does anyone has the experience with HiYield Gel/PCR DNA Fragment kit and HiYield Plasmid Mini kit from RBC Bioscience?

I need to extract 101 bp DNA fragment from 3% agarose gel for cloning. Then, after cloning procedure, I need to extract the plasmid for sequencing. Thus, I want to know if these extraction kits...

08 January 2018 2,823 2 View

Is there a way to solve the problem of "data below minimum" in my plotting?

And what does it mean when the first two peaks of my titration are endothermic? I'm titrating a substrate to a protein and it was supposed to bind but not react. The MicroCal PEAQ analysis tell me...

25 April 2016 2,676 1 View

Hi! Is there any software or app to built Venn Diagrams for big data sets (around 39000 elements in each set)?

01 October 2015 944 3 View

Why do I have a low RNA concentration using Trizol?

09 June 2014 7,318 3 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Do anyone here can help us in the field of molecular docking?

We have synthesized various metal complexes with different environment around the metals. Now to see the application of synthesized complexes we need this study also. we are also interested in...

01 March 2021 1,899 2 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Can chitosan alone can act as a reducing agent for Cu(0) nanoparticle sythesis?

[Synthesizing Cu nanoparticles without using chemical reductants]

01 March 2021 8,359 1 View

What are the new 2D nanomaterials were used for Silver nanoparticles stabilization?

In the stabilization of AgNPs are vital in current research interest. However, some of the 2D nano-materials like graphene oxide, MoS2, etc... previously used to stabilize it. But still they shown...

01 March 2021 2,207 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

How to check the uv-vis absorption of nanoparticle by surface plasmon resonance?

I try to check the uv-vis absorption of Indium nanoparticle. Now I am measuring in solution, can I measure it in the form of a film?

01 March 2021 930 3 View

Distribution of particles in metal matrix?

How would define the "good" and "poor" distribution of particles within the matrix. Is there a quantitative solution to assess the distribution of the particles, which then having denotation of...

28 February 2021 7,397 8 View

Can anyone help me with the administration of the "Mind the Gap" Scale for satisfaction with transition to adult care?

We are working on a study focused on adolescents' transition to adult care and are looking for a validated measure for satisfaction with the transition. So far the only measure that we have came...

28 February 2021 2,338 1 View