What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

Which isomer of xylene do you use in hystology ?

Which isomer of xylene do you use for the preparation of histological tissue sections - meta para or ortho?

02 February 2021 833 4 View

Can we re-freezing of brain tissue homogenates?

Hello! We use homogenates of mouse brains for our aims (determination of cytokines by the ELISA method). After removing the brain, we freeze the tissue in liquid nitrogen, then store it for a...

19 January 2021 5,203 1 View

What is a functional blocking assay for an antibody fraction?

I will be grateful if you could explain me or give a reference where I can read about: - What steps a functional blocking assay could include? - Are there other possibilities to prove presence...

17 November 2020 3,032 3 View

Should antibodies against the receptor binding domain of a spike protein bind to a complete spike protein?

The receptor binding domain of the coronavirus spike protein has been expressed in Escherichia coli. Antibodies were obtained to the purified protein. These antibodies do not recognize the spike...

13 October 2020 327 5 View

How to perform overlay from different experiments in FACS Diva?

Dear colleagues! I am in desperate need to do overlay data collected on different days in FACS Diva (need to compare some markers expression levels on different days of cell growth). The settings...

28 July 2020 1,779 3 View

How to correctly choose population strata and interpret results for the detection of loci under selection from RADseq data using Bayescan?

I am working on the analysis of fungal populations sampled from 12 geographic locations using RADseq data. The dataset contains 7439 polymorphic loci with ~10 snps per RAD locus (75,482 snps...

19 May 2020 643 3 View

Good entry literature on philosophy and/ or ethics of technology?

Hello, I am searching for a good introduction or overview on the topics, methods and research of philosophy or ethics of technology (separately). Any suggestions? Thanks in advance! Hallo, suche...

23 April 2020 5,096 1 View

What will be the real consequences of the COVID-19 pandemic on corporate credit rating?

We know that with Basel all banks adopt a rating system to evaluate their loans and to calculate their regulatory capital. companies have always had problems with the rating calculated by banks...

08 April 2020 9,034 7 View

Editing experimentsl and control cells with the lentivirus, I have prevailing signals from cell immune response on virus. How can that be?

Hello everybody! We have modified cells with the lentiviral construction and then analyzed the result by RNAseq. I've made gene set enrichment analysis in R-package fgsea. And obtained the result,...

03 March 2020 9,876 1 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

What is the significance of (isolated) high titers of IgG anti GD1a antibodies in an older patient with polyneuropathy?

Remote history of Campylobacter infection with reactive arthritis, and Spitz nevus (UMP). Arthropod exposure in Zika/ChikV endemic area prior to emergence of length dependent, sensory PN with...

18 February 2021 8,719 1 View

What is the cause of a smear from direct DNA amplification from human buccal swab samples ?

Dear fellow scientists, I like to performed a direct amplification of a fragment of 222-bp DNA from human genome and use is for further analysis. By using the following methods, I obtained...

07 February 2021 891 3 View

Is it strictly recommended to run samples in duplicate during SNP genotyping analysis?

02 February 2021 8,877 3 View

Help needed: How to map AA, AG, and GG genotypes on to polymorphism information?

Dear Colleagues, I am new to genes and genotyping and I need help. I am trying to figure our how AA, AG, and GG genotypes map on to A1 for DRD2/ANKK1 Taq1A polymorphism(rs1800497) and Val or MET...

02 February 2021 3,861 3 View

How to remove colour from DNA pellet during DNA extraction?

I am working with Amaranth accessions which are green to red in color and contain high phenolic compounds. Using the CTAB method with the slight modification I am trying to get high-quality DNA so...

18 January 2021 3,578 1 View

How do I get ConsistencyDupSNP.sh file required for genotype consistency quality control step of genotyping data obtained from GSA 24 v2.0 ?

Genotyping data is from Illumina Global screening array 24 v 2.0 and ConsistencyDupSNP.sh file contains list of pair of duplicated SNPs in GSA24 v 2.0.

18 December 2020 1,966 3 View

Why my PCR started to show extra bands and weird results?

Hello guys, I need your help. I used to do PCR for genotyping of my LCK Cre mice. The PCR was working well. You can see in Fig A (Attached picture). No template NT was clean and negative control...

26 November 2020 4,765 3 View

What's happened to my abnormal realtime pcr amplification curve?

I am working on quantstudio 5 system with diagnostic test kit - AZF Microdeletions REALTIME PCR genotyping kit (master mix is aliquoted to every separated pcr tube, I only added taq polymerase,...

19 November 2020 4,840 3 View